| Title |
Expression of antifreeze proteins in transgenic plants
|
|---|---|
| Published in |
Plant Molecular Biology, November 1991
|
| DOI | 10.1007/bf00037141 |
| Pubmed ID | |
| Authors |
Robin Hightower, Cathy Baden, Eva Penzes, Peter Lund, Pamela Dunsmuir |
| Abstract |
The quality of frozen fruits and vegetables can be compromised by the damaging effects of ice crystal growth within the frozen tissue. Antifreeze proteins in the blood of some polar fishes have been shown to inhibit ice recrystallization at low concentrations. In order to determine whether expression of genes of this type confers improved freezing properties to plant tissue, we have produced transgenic tobacco and tomato plants which express genes encoding antifreeze proteins. The afa3 antifreeze gene was expressed at high steady-state mRNA levels in leaves from transformed plants, but we did not detect inhibition of ice recrystallization in tissue extracts. However, both mRNA and fusion proteins were detectable in transgenic tomato tissue containing a chimeric gene encoding a fusion protein truncated staphylococcal protein A and antifreeze protein. Furthermore, ice recrystallization inhibition was detected in this transgenic tissue. |
X Demographics
The data shown below were collected from the profiles of 16 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | 25% |
| Japan | 2 | 13% |
| Peru | 2 | 13% |
| Germany | 1 | 6% |
| Canada | 1 | 6% |
| France | 1 | 6% |
| Unknown | 5 | 31% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 14 | 88% |
| Scientists | 2 | 13% |
Mendeley readers
The data shown below were compiled from readership statistics for 49 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 49 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 12 | 24% |
| Student > Master | 8 | 16% |
| Researcher | 6 | 12% |
| Student > Ph. D. Student | 6 | 12% |
| Professor > Associate Professor | 3 | 6% |
| Other | 5 | 10% |
| Unknown | 9 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 20 | 41% |
| Biochemistry, Genetics and Molecular Biology | 7 | 14% |
| Chemistry | 4 | 8% |
| Earth and Planetary Sciences | 2 | 4% |
| Immunology and Microbiology | 1 | 2% |
| Other | 3 | 6% |
| Unknown | 12 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 139. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 June 2023.
All research outputs
#281,233
of 24,417,324 outputs
Outputs from Plant Molecular Biology
#2
of 2,884 outputs
Outputs of similar age
#35
of 19,187 outputs
Outputs of similar age from Plant Molecular Biology
#1
of 36 outputs
Altmetric has tracked 24,417,324 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 98th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,884 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done particularly well, scoring higher than 99% of its peers.
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We're also able to compare this research output to 36 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.