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Single‐Molecule Force Spectroscopy Trajectories of a Single Protein and Its Polyproteins Are Equivalent: A Direct Experimental Validation Based on A Small Protein NuG2

Overview of attention for article published in Angewandte Chemie. International Edition, December 2016
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Title
Single‐Molecule Force Spectroscopy Trajectories of a Single Protein and Its Polyproteins Are Equivalent: A Direct Experimental Validation Based on A Small Protein NuG2
Published in
Angewandte Chemie. International Edition, December 2016
DOI 10.1002/anie.201610648
Pubmed ID
Authors

Hai Lei, Chengzhi He, Chunguang Hu, Jinliang Li, Xiaodong Hu, Xiaotang Hu, Hongbin Li

Abstract

Single-molecule force spectroscopy (SMFS) has become a powerful tool in investigating the mechanical unfolding/folding of proteins at the single-molecule level. Polyproteins made of tandem identical repeats have been widely used in atomic force microscopy (AFM)-based SMFS studies, where polyproteins not only serve as fingerprints to identify single-molecule stretching events, but may also improve statistics of data collection. However, the inherent assumption of such experiments is that all the domains in the polyprotein are equivalent and one SMFS trajectory of stretching a polyprotein made of n domains is equivalent to n trajectories of stretching a single domain. Such an assumption has not been validated experimentally. Using a small protein NuG2 and its polyprotein (NuG2)4 as model systems, here we use optical trapping (OT) to directly validate this assumption. Our results show that OT experiments on NuG2 and (NuG2)4 lead to identical parameters describing the unfolding and folding kinetics of NuG2, demonstrating that indeed stretching a polyprotein of NuG2 is equivalent to stretching single NuG2 in force spectroscopy experiments and thus validating the use of polyproteins in SMFS experiments.

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Geographical breakdown

Country Count As %
Bangladesh 1 4%
Germany 1 4%
Unknown 23 92%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 28%
Student > Master 5 20%
Student > Bachelor 4 16%
Professor 2 8%
Researcher 2 8%
Other 2 8%
Unknown 3 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 24%
Biochemistry, Genetics and Molecular Biology 4 16%
Physics and Astronomy 4 16%
Chemistry 3 12%
Engineering 2 8%
Other 3 12%
Unknown 3 12%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 December 2016.
All research outputs
#22,758,309
of 25,373,627 outputs
Outputs from Angewandte Chemie. International Edition
#45,795
of 49,982 outputs
Outputs of similar age
#362,863
of 422,416 outputs
Outputs of similar age from Angewandte Chemie. International Edition
#660
of 724 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 49,982 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.8. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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