| Title |
Antimicrobial Resistance, Virulence Genes, and Genetic Lineages of Staphylococcus pseudintermedius in Healthy Dogs in Tunisia
|
|---|---|
| Published in |
Microbial Ecology, May 2013
|
| DOI | 10.1007/s00248-013-0243-y |
| Pubmed ID | |
| Authors |
Haythem Gharsa, Karim Ben Slama, Elena Gómez-Sanz, Carmen Lozano, Naouel Klibi, Ahlem Jouini, Lilia Messadi, Abdellatif Boudabous, Carmen Torres |
| Abstract |
Nasal swabs of 100 healthy dogs were obtained in 2011 in Tunisia and tested for Staphylococcus pseudintermedius recovery. Antimicrobial resistance profile and virulence gene content were determined. Multilocus-sequence-typing (MLST) and SmaI-pulsed-field gel electrophoresis (PFGE) were investigated. S. pseudintermedius was recovered in 55 of the 100 tested samples (55 %), and one isolate per sample was further studied. All 55 S. pseudintermedius isolates were susceptible to methicillin (MSSP) but showed resistance to the following antimicrobials (% resistant isolates/resistance gene): penicillin (56.4/blaZ), tetracycline (40/tetM), trimethoprim-sulfamethoxazole (23.7), fusidic acid (9), kanamycin (3.7/aph(3´)-Ia), erythromycin-clindamycin (1.8/erm(B)), streptomycin (1.8/ant(6)-Ia), chloramphenicol (1.8) and ciprofloxacin (1.8). The following toxin genes were identified (% of isolates): lukS/F-I (98.2), expA (5.5), se-int (98.2), sec canine (1.8), siet (100), sea (5.5), seb (3.6), sec (10.9), sed (54.5), sei (5.5), sej (29.1), sek (3.6), ser (9.1), and hlg v (38.2). Ten different sequence-types were detected among 11 representative MSSP isolates: ST20, ST44, ST69, ST70, ST78, ST100, ST108, ST160, ST161, and ST162, the last three ones revealing novel alleles or allele combinations. Eleven different PFGE-patterns were identified in these isolates. The nares of healthy dogs could be a reservoir of antimicrobial resistant and virulent MSSP, highlighting the presence of the recently described exfoliating gene expA and several enterotoxin genes. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Argentina | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 63 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Sri Lanka | 1 | 2% |
| France | 1 | 2% |
| Brazil | 1 | 2% |
| Unknown | 60 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 10 | 16% |
| Student > Bachelor | 7 | 11% |
| Researcher | 6 | 10% |
| Student > Ph. D. Student | 6 | 10% |
| Professor | 5 | 8% |
| Other | 18 | 29% |
| Unknown | 11 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 13 | 21% |
| Medicine and Dentistry | 9 | 14% |
| Immunology and Microbiology | 9 | 14% |
| Agricultural and Biological Sciences | 8 | 13% |
| Biochemistry, Genetics and Molecular Biology | 4 | 6% |
| Other | 5 | 8% |
| Unknown | 15 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 May 2013.
All research outputs
#15,222,208
of 22,711,242 outputs
Outputs from Microbial Ecology
#1,456
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Outputs of similar age
#121,062
of 196,556 outputs
Outputs of similar age from Microbial Ecology
#12
of 16 outputs
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