| Title |
Characterization and genome analysis of novel bacteriophages infecting the opportunistic human pathogens Klebsiella oxytoca and K. pneumoniae
|
|---|---|
| Published in |
Archives of Virology, December 2016
|
| DOI | 10.1007/s00705-016-3202-3 |
| Pubmed ID | |
| Authors |
Eun-Ah Park, You-Tae Kim, Jae-Hyun Cho, Sangryeol Ryu, Ju-Hoon Lee |
| Abstract |
Klebsiella is a genus of well-known opportunistic human pathogens that are associated with diabetes mellitus and chronic pulmonary obstruction; however, this pathogen is often resistant to multiple drugs. To control this pathogen, two Klebsiella-infecting phages, K. oxytoca phage PKO111 and K. pneumoniae phage PKP126, were isolated from a sewage sample. Analysis of their host range revealed that they infect K. pneumoniae and K. oxytoca, suggesting host specificity for members of the genus Klebsiella. Stability tests confirmed that the phages are stable under various temperature (4 to 60 °C) and pH (3 to 11) conditions. A challenge assay showed that PKO111 and PKP126 inhibit growth of their host strains by 2 log and 4 log, respectively. Complete genome sequencing of the phages revealed that their genome sizes are quite different (168,758 bp for PKO111 and 50,934 bp for PKP126). Their genome annotation results showed that they have no human virulence-related genes, an important safety consideration. In addition, no lysogen-formation gene cluster was detected in either phage genome, suggesting that they are both virulent phages in their bacterial hosts. Based on these results, PKO111 and PKP126 may be good candidates for development of biocontrol agents against members of the genus Klebsiella for therapeutic purposes. A comparative analysis of tail-associated gene clusters of PKO111 and PKP126 revealed relatively low homology, suggesting that they might differ in the way they recognize and infect their specific hosts. |
Mendeley readers
The data shown below were compiled from readership statistics for 47 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 2% |
| United States | 1 | 2% |
| Unknown | 45 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 10 | 21% |
| Student > Bachelor | 9 | 19% |
| Student > Ph. D. Student | 7 | 15% |
| Researcher | 6 | 13% |
| Other | 2 | 4% |
| Other | 4 | 9% |
| Unknown | 9 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 10 | 21% |
| Agricultural and Biological Sciences | 9 | 19% |
| Biochemistry, Genetics and Molecular Biology | 8 | 17% |
| Medicine and Dentistry | 6 | 13% |
| Veterinary Science and Veterinary Medicine | 2 | 4% |
| Other | 2 | 4% |
| Unknown | 10 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 December 2016.
All research outputs
#15,416,191
of 22,925,760 outputs
Outputs from Archives of Virology
#2,612
of 4,198 outputs
Outputs of similar age
#256,462
of 420,925 outputs
Outputs of similar age from Archives of Virology
#21
of 40 outputs
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So far Altmetric has tracked 4,198 research outputs from this source. They receive a mean Attention Score of 3.7. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
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