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Identification of Unsafe Human Induced Pluripotent Stem Cell Lines Using a Robust Surrogate Assay for Pluripotency

Overview of attention for article published in Stem Cells, August 2013
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (97th percentile)
  • High Attention Score compared to outputs of the same age and source (94th percentile)

Mentioned by

news
6 news outlets
blogs
2 blogs
twitter
4 X users
facebook
1 Facebook page

Citations

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22 Dimensions

Readers on

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50 Mendeley
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Title
Identification of Unsafe Human Induced Pluripotent Stem Cell Lines Using a Robust Surrogate Assay for Pluripotency
Published in
Stem Cells, August 2013
DOI 10.1002/stem.1425
Pubmed ID
Authors

Juan Carlos Polanco, Mirabelle S.H. Ho, Bei Wang, Qi Zhou, Ernst Wolvetang, Elizabeth Mason, Christine A. Wells, Gabriel Kolle, Sean M. Grimmond, Ivan Bertoncello, Carmel O'Brien, Andrew L. Laslett

Abstract

Human induced pluripotent stem cells (hiPSC) have the potential to generate healthy cells and tissues for the study and medical treatment of a large number of diseases. The utility of putative hiPSC-based therapies is constrained by a lack of robust quality-control assays that address the stability of the cells or their capacity to form teratomas after differentiation. Here we report that virally derived hiPSC, but not human embryonic stem cells (hESC) or hiPSC derived using episomal nonintegrating vectors, exhibit a propensity to revert to a pluripotent phenotype following differentiation. This instability was revealed using our published method to identify pluripotent cells undergoing very early-stage differentiation in standard hESC cultures, by fluorescence activated cell sorting (FACS) based on expression of the cell surface markers TG30 (CD9) and GCTM-2. Differentiated cells cultured post-FACS fractionation from virally derived hiPSC lines reacquired immunoreactivity to TG30 (CD9) and GCTM-2, formed stem cell-like colonies, and re-expressed canonical pluripotency markers. Furthermore, differentiated cells from pluripotency-reverting hiPSC lines generated teratomas in immunocompromised mice, raising concerns about their safety in downstream applications. In contrast, differentiated cell populations from hESC and episomally derived hiPSC did not show any of these abnormalities. Our assays may be used to identify "unsafe" hiPSC cell lines and this information should be considered when selecting hiPSC lines for clinical use and indicate that experiments using these "unsafe" hiPSC lines should be interpreted carefully.

X Demographics

X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 50 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 6%
Iran, Islamic Republic of 1 2%
France 1 2%
Brazil 1 2%
Unknown 44 88%

Demographic breakdown

Readers by professional status Count As %
Researcher 17 34%
Student > Ph. D. Student 13 26%
Professor > Associate Professor 5 10%
Student > Master 5 10%
Student > Bachelor 2 4%
Other 5 10%
Unknown 3 6%
Readers by discipline Count As %
Agricultural and Biological Sciences 23 46%
Biochemistry, Genetics and Molecular Biology 10 20%
Medicine and Dentistry 5 10%
Materials Science 3 6%
Engineering 2 4%
Other 3 6%
Unknown 4 8%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 59. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 June 2013.
All research outputs
#605,062
of 22,711,645 outputs
Outputs from Stem Cells
#74
of 3,897 outputs
Outputs of similar age
#5,374
of 199,020 outputs
Outputs of similar age from Stem Cells
#2
of 38 outputs
Altmetric has tracked 22,711,645 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,897 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.7. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 199,020 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 97% of its contemporaries.
We're also able to compare this research output to 38 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.