| Title |
Colonization of Madagascar periwinkle (Catharanthus roseus), by endophytes encoding gfp marker
|
|---|---|
| Published in |
Archives of Microbiology, May 2013
|
| DOI | 10.1007/s00203-013-0897-3 |
| Pubmed ID | |
| Authors |
Adalgisa Ribeiro Torres, Welington Luiz Araújo, Luciana Cursino, Priscilla de Barros Rossetto, Mateus Mondin, Mariangela Hungria, João Lúcio Azevedo |
| Abstract |
This study reports the introduction of gfp marker in two endophytic bacterial strains (Pantoea agglomerans C33.1, isolated from cocoa, and Enterobacter cloacae PR2/7, isolated from citrus) to monitor the colonization in Madagascar perinwinkle (Catharanthus roseus). Stability of the plasmid encoding gfp was confirmed in vitro for at least 72 h of bacterial growth and after the colonization of tissues, under non-selective conditions. The colonization was observed using fluorescence microscopy and enumeration of culturable endophytes in inoculated perinwinkle plants that grew for 10 and 20 days. Gfp-expressing strains were re-isolated from the inner tissues of surface-sterilized roots and stems of inoculated plants, and the survival of the P. agglomerans C33:1gfp in plants 20 days after inoculation, even in the absence of selective pressure, suggests that is good colonizer. These results indicated that both gfp-tagged strains, especially P. agglomerans C33.1, may be useful tools to deliver enzymes or other proteins in plant. |
Mendeley readers
The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 3% |
| India | 1 | 3% |
| Brazil | 1 | 3% |
| Unknown | 29 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 5 | 16% |
| Researcher | 4 | 13% |
| Student > Ph. D. Student | 3 | 9% |
| Professor | 3 | 9% |
| Student > Master | 3 | 9% |
| Other | 6 | 19% |
| Unknown | 8 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 16 | 50% |
| Environmental Science | 3 | 9% |
| Biochemistry, Genetics and Molecular Biology | 1 | 3% |
| Unspecified | 1 | 3% |
| Immunology and Microbiology | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 9 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 June 2013.
All research outputs
#20,194,368
of 22,711,645 outputs
Outputs from Archives of Microbiology
#2,335
of 2,762 outputs
Outputs of similar age
#170,555
of 195,600 outputs
Outputs of similar age from Archives of Microbiology
#3
of 3 outputs
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So far Altmetric has tracked 2,762 research outputs from this source. They receive a mean Attention Score of 3.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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