| Title |
Enhanced succinic acid production in Aspergillus saccharolyticus by heterologous expression of fumarate reductase from Trypanosoma brucei
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, October 2015
|
| DOI | 10.1007/s00253-015-7086-z |
| Pubmed ID | |
| Authors |
Lei Yang, Mette Lübeck, Birgitte K. Ahring, Peter S. Lübeck |
| Abstract |
Aspergillus saccharolyticus exhibits great potential as a cell factory for industrial production of dicarboxylic acids. In the analysis of the organic acid profile, A. saccharolyticus was cultivated in an acid production medium using two different pH conditions. The specific activities of the enzymes, pyruvate carboxylase (PYC), malate dehydrogenase (MDH), and fumarase (FUM), involved in the reductive tricarboxylic acid (rTCA) branch, were examined and compared in cells harvested from the acid production medium and a complete medium. The results showed that ambient pH had a significant impact on the pattern and the amount of organic acids produced by A. saccharolyticus. The wild-type strain produced higher amount of malic acid and succinic acid in the pH buffered condition (pH 6.5) compared with the pH non-buffered condition. The enzyme assays showed that the rTCA branch was active in the acid production medium as well as the complete medium, but the measured enzyme activities were different depending on the media. Furthermore, a soluble NADH-dependent fumarate reductase gene (frd) from Trypanosoma brucei was inserted and expressed in A. saccharolyticus. The expression of the frd gene led to an enhanced production of succinic acid in frd transformants compared with the wild-type in both pH buffered and pH non-buffered conditions with highest amount produced in the pH buffered condition (16.2 ± 0.5 g/L). This study demonstrates the feasibility of increasing succinic acid production through the cytosolic reductive pathway by genetic engineering in A. saccharolyticus. |
Mendeley readers
The data shown below were compiled from readership statistics for 53 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Ghana | 1 | 2% |
| Unknown | 52 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 9 | 17% |
| Student > Master | 9 | 17% |
| Student > Ph. D. Student | 8 | 15% |
| Student > Doctoral Student | 3 | 6% |
| Researcher | 3 | 6% |
| Other | 7 | 13% |
| Unknown | 14 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 13 | 25% |
| Agricultural and Biological Sciences | 12 | 23% |
| Engineering | 5 | 9% |
| Chemistry | 4 | 8% |
| Chemical Engineering | 3 | 6% |
| Other | 2 | 4% |
| Unknown | 14 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 January 2017.
All research outputs
#8,882,501
of 26,017,215 outputs
Outputs from Applied Microbiology and Biotechnology
#3,054
of 8,484 outputs
Outputs of similar age
#104,762
of 297,831 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#36
of 141 outputs
Altmetric has tracked 26,017,215 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 8,484 research outputs from this source. They receive a mean Attention Score of 4.5. This one is in the 34th percentile – i.e., 34% of its peers scored the same or lower than it.
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We're also able to compare this research output to 141 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 63% of its contemporaries.