| Title |
Yeast cells as microcapsules. Analytical tools and process variables in the encapsulation of hydrophobes in S. cerevisiae
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, May 2012
|
| DOI | 10.1007/s00253-012-4127-8 |
| Pubmed ID | |
| Authors |
Federica Ciamponi, Craig Duckham, Nicola Tirelli |
| Abstract |
Yeast cells can be used as biocompatible and biodegradable containers for the microencapsulation of a variety of actives. Despite the wide application of this process, e.g. in the food industry, mechanism and controlling factors are yet poorly known. In this study we have studied kinetics and mechanistic aspects of the spontaneous internalization of terpenes (as model hydrophobic compounds) in Saccharomyces cerevisiae, quantifying their encapsulation through HPLC analysis and fluorescent staining of lipidic bodies with Nile Red, while in parallel monitoring cell viability. Our results showed that this encapsulation process is essentially a phenomenon of passive diffusion with negligible relevance of active transport. Further, our evidence shows that the major determinant of the encapsulation kinetics is the solubility of the hydrophobe in the cell wall, which is inversely related to partition coefficient (log P). |
Mendeley readers
The data shown below were compiled from readership statistics for 75 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 1% |
| Australia | 1 | 1% |
| Canada | 1 | 1% |
| Unknown | 72 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 18 | 24% |
| Researcher | 13 | 17% |
| Student > Master | 11 | 15% |
| Student > Bachelor | 9 | 12% |
| Student > Doctoral Student | 2 | 3% |
| Other | 6 | 8% |
| Unknown | 16 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 20 | 27% |
| Biochemistry, Genetics and Molecular Biology | 10 | 13% |
| Engineering | 5 | 7% |
| Chemistry | 5 | 7% |
| Chemical Engineering | 4 | 5% |
| Other | 9 | 12% |
| Unknown | 22 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 May 2019.
All research outputs
#4,986,314
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#1,213
of 8,034 outputs
Outputs of similar age
#32,815
of 166,502 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#14
of 75 outputs
Altmetric has tracked 24,119,703 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,034 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 166,502 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 75 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 81% of its contemporaries.