| Title |
Expression and characterization of HSPC129, a RNA polymerase II C-terminal domain phosphatase
|
|---|---|
| Published in |
Molecular and Cellular Biochemistry, May 2007
|
| DOI | 10.1007/s11010-007-9472-z |
| Pubmed ID | |
| Authors |
Hui Qian, Chaoneng Ji, Shuo Zhao, Jinzhong Chen, Mei Jiang, Yong Zhang, Mi Yan, Dan Zheng, Yaqiong Sun, Yi Xie, Yumin Mao |
| Abstract |
Phosphorylation status of RNA polymerase (RNAP) II's largest subunit C-terminal domain (CTD) plays an important role during transcription cycles. The reversible phosphorylation mainly occurs at serine 2 and serine 5 of CTD heptapeptide repeats and regulates RNAP II's activity during transcription initiation, elongation and RNA processing. Here we expressed and characterized HSPC129, a putative human protein bearing a CTD phosphatase domain (CPD). PCR analysis showed that it was ubiquitously expressed. HSPC129DeltaTM, the truncate HSPC129 with first 156 N terminal amino acids deleted, exhibited Mg(2+) dependent phosphatase activity at pH 5.0. Its specific CTD phosphatase activity was verified in vitro. Our research suggests that HSPC129 may regulate the dynamic phosphorylation of RNAP II CTD. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 33% |
| Student > Ph. D. Student | 3 | 25% |
| Professor | 2 | 17% |
| Lecturer > Senior Lecturer | 1 | 8% |
| Other | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 1 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 33% |
| Biochemistry, Genetics and Molecular Biology | 2 | 17% |
| Environmental Science | 1 | 8% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
| Computer Science | 1 | 8% |
| Other | 2 | 17% |
| Unknown | 1 | 8% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 October 2019.
All research outputs
#15,272,977
of 22,711,645 outputs
Outputs from Molecular and Cellular Biochemistry
#1,316
of 2,292 outputs
Outputs of similar age
#62,144
of 71,922 outputs
Outputs of similar age from Molecular and Cellular Biochemistry
#11
of 13 outputs
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We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one is in the 7th percentile – i.e., 7% of its contemporaries scored the same or lower than it.