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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Pooled CRISPR screening with single-cell transcriptome readout
|
|---|---|
| Published in |
Nature Methods, January 2017
|
| DOI | 10.1038/nmeth.4177 |
| Pubmed ID | |
| Authors |
Paul Datlinger, André F Rendeiro, Christian Schmidl, Thomas Krausgruber, Peter Traxler, Johanna Klughammer, Linda C Schuster, Amelie Kuchler, Donat Alpar, Christoph Bock |
| Abstract |
CRISPR-based genetic screens are accelerating biological discovery, but current methods have inherent limitations. Widely used pooled screens are restricted to simple readouts including cell proliferation and sortable marker proteins. Arrayed screens allow for comprehensive molecular readouts such as transcriptome profiling, but at much lower throughput. Here we combine pooled CRISPR screening with single-cell RNA sequencing into a broadly applicable workflow, directly linking guide RNA expression to transcriptome responses in thousands of individual cells. Our method for CRISPR droplet sequencing (CROP-seq) enables pooled CRISPR screens with single-cell transcriptome resolution, which will facilitate high-throughput functional dissection of complex regulatory mechanisms and heterogeneous cell populations. |
X Demographics
The data shown below were collected from the profiles of 88 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 25 | 28% |
| Austria | 10 | 11% |
| United Kingdom | 10 | 11% |
| Switzerland | 3 | 3% |
| Netherlands | 2 | 2% |
| Canada | 2 | 2% |
| France | 2 | 2% |
| Belgium | 2 | 2% |
| Germany | 2 | 2% |
| Other | 7 | 8% |
| Unknown | 23 | 26% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 45 | 51% |
| Scientists | 41 | 47% |
| Science communicators (journalists, bloggers, editors) | 2 | 2% |
Mendeley readers
The data shown below were compiled from readership statistics for 1,978 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 7 | <1% |
| Switzerland | 2 | <1% |
| Austria | 2 | <1% |
| United Kingdom | 2 | <1% |
| South Africa | 1 | <1% |
| Israel | 1 | <1% |
| Sweden | 1 | <1% |
| Belgium | 1 | <1% |
| Germany | 1 | <1% |
| Other | 2 | <1% |
| Unknown | 1958 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 442 | 22% |
| Researcher | 413 | 21% |
| Student > Master | 175 | 9% |
| Student > Bachelor | 166 | 8% |
| Student > Postgraduate | 77 | 4% |
| Other | 265 | 13% |
| Unknown | 440 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 695 | 35% |
| Agricultural and Biological Sciences | 396 | 20% |
| Immunology and Microbiology | 77 | 4% |
| Medicine and Dentistry | 72 | 4% |
| Neuroscience | 66 | 3% |
| Other | 198 | 10% |
| Unknown | 474 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 155. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 March 2024.
All research outputs
#269,752
of 26,017,215 outputs
Outputs from Nature Methods
#265
of 5,404 outputs
Outputs of similar age
#5,718
of 428,081 outputs
Outputs of similar age from Nature Methods
#6
of 93 outputs
Altmetric has tracked 26,017,215 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 98th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 5,404 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 36.8. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 428,081 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 98% of its contemporaries.
We're also able to compare this research output to 93 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.