| Title |
Using Protein Misfolding Cyclic Amplification Generates a Highly Neurotoxic PrP Dimer Causing Neurodegeneration
|
|---|---|
| Published in |
Journal of Molecular Neuroscience, June 2013
|
| DOI | 10.1007/s12031-013-0039-z |
| Pubmed ID | |
| Authors |
XiuJin Yang, LiFeng Yang, XiangMei Zhou, Sher Hayat Khan, HuiNuan Wang, XiaoMin Yin, Zhen Yuan, ZhiQi Song, WenYu Wu, DeMing Zhao |
| Abstract |
Under the "protein-only" hypothesis, prion-based diseases are proposed to result from an infectious agent that is an abnormal isoform of the prion protein in the scrapie form, PrP(Sc). However, since PrP(Sc) is highly insoluble and easily aggregates in vivo, this view appears to be overly simplistic, implying that the presence of PrP(Sc) may indirectly cause neurodegeneration through its intermediate soluble form. We generated a neurotoxic PrP dimer with partial pathogenic characteristics of PrP(Sc) by protein misfolding cyclic amplification in the presence of 1-palmitoyl-2-oleoylphosphatidylglycerol consisting of recombinant hamster PrP (23-231). After intracerebral injection of the PrP dimer, wild-type hamsters developed signs of neurodegeneration. Clinical symptoms, necropsy findings, and histopathological changes were very similar to those of transmissible spongiform encephalopathies. Additional investigation showed that the toxicity is primarily related to cellular apoptosis. All results suggested that we generated a new neurotoxic form of PrP, PrP dimer, which can cause neurodegeneration. Thus, our study introduces a useful model for investigating PrP-linked neurodegenerative mechanisms. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| China | 1 | 5% |
| Unknown | 20 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 48% |
| Professor > Associate Professor | 3 | 14% |
| Professor | 2 | 10% |
| Lecturer > Senior Lecturer | 1 | 5% |
| Other | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 2 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 33% |
| Agricultural and Biological Sciences | 7 | 33% |
| Nursing and Health Professions | 1 | 5% |
| Immunology and Microbiology | 1 | 5% |
| Psychology | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 2 | 10% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 June 2013.
All research outputs
#19,944,994
of 25,374,647 outputs
Outputs from Journal of Molecular Neuroscience
#1,094
of 1,643 outputs
Outputs of similar age
#143,991
of 197,430 outputs
Outputs of similar age from Journal of Molecular Neuroscience
#13
of 27 outputs
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