| Title |
Acute low-intensity cycling with blood-flow restriction has no effect on metabolic signaling in human skeletal muscle compared to traditional exercise
|
|---|---|
| Published in |
European Journal of Applied Physiology, January 2017
|
| DOI | 10.1007/s00421-016-3530-8 |
| Pubmed ID | |
| Authors |
William J. Smiles, Miguel S. Conceição, Guilherme D. Telles, Mara P. T. Chacon-Mikahil, Cláudia R. Cavaglieri, Felipe C. Vechin, Cleiton A. Libardi, John A. Hawley, Donny M. Camera |
| Abstract |
Autophagy is an intracellular degradative system sensitive to hypoxia and exercise-induced perturbations to cellular bioenergetics. We determined the effects of low-intensity endurance-based exercise performed with blood-flow restriction (BFR) on cell signaling adaptive responses regulating autophagy and substrate metabolism in human skeletal muscle. In a randomized cross-over design, nine young, healthy but physically inactive males completed three experimental trials separated by 1 week of recovery consisting of either a resistance exercise bout (REX: 4 × 10 leg press repetitions, 70% 1-RM), endurance exercise (END: 30 min cycling, 70% VO2peak), or low-intensity cycling with BFR (15 min, 40% VO2peak). A resting muscle biopsy was obtained from the vastus lateralis 2 weeks prior to the first exercise trial and 3 h after each exercise bout. END increased ULK1(Ser757) phosphorylation above rest and BFR (~37 to 51%, P < 0.05). Following REX, there were significant elevations compared to rest (~348%) and BFR (~973%) for p38γ MAPK(Thr180/Tyr182) phosphorylation (P < 0.05). Parkin content was lower following BFR cycling compared to REX (~20%, P < 0.05). There were no exercise-induced changes in select markers of autophagy following BFR. Genes implicated in substrate metabolism (HK2 and PDK4) were increased above rest (~143 to 338%) and BFR cycling (~212 to 517%) with END (P < 0.001). A single bout of low-intensity cycling with BFR is insufficient to induce intracellular "stress" responses (e.g., high rates of substrate turnover and local hypoxia) necessary to activate skeletal muscle autophagy signaling. |
X Demographics
The data shown below were collected from the profiles of 22 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 5 | 23% |
| United Kingdom | 3 | 14% |
| Australia | 2 | 9% |
| Canada | 1 | 5% |
| Sweden | 1 | 5% |
| Ireland | 1 | 5% |
| Unknown | 9 | 41% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 11 | 50% |
| Scientists | 9 | 41% |
| Practitioners (doctors, other healthcare professionals) | 2 | 9% |
Mendeley readers
The data shown below were compiled from readership statistics for 181 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | <1% |
| Colombia | 1 | <1% |
| Brazil | 1 | <1% |
| Unknown | 178 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 29 | 16% |
| Student > Bachelor | 28 | 15% |
| Student > Ph. D. Student | 15 | 8% |
| Student > Doctoral Student | 12 | 7% |
| Researcher | 11 | 6% |
| Other | 27 | 15% |
| Unknown | 59 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Sports and Recreations | 51 | 28% |
| Nursing and Health Professions | 21 | 12% |
| Medicine and Dentistry | 20 | 11% |
| Psychology | 6 | 3% |
| Agricultural and Biological Sciences | 4 | 2% |
| Other | 12 | 7% |
| Unknown | 67 | 37% |
Attention Score in Context
This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 April 2017.
All research outputs
#3,223,164
of 25,377,790 outputs
Outputs from European Journal of Applied Physiology
#973
of 4,345 outputs
Outputs of similar age
#61,948
of 422,427 outputs
Outputs of similar age from European Journal of Applied Physiology
#20
of 53 outputs
Altmetric has tracked 25,377,790 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,345 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 14.7. This one has done well, scoring higher than 77% of its peers.
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We're also able to compare this research output to 53 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 62% of its contemporaries.