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Evolution of Substrate Specificity in a Recipient’s Enzyme Following Horizontal Gene Transfer

Overview of attention for article published in Molecular Biology and Evolution, June 2013
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (73rd percentile)
  • Above-average Attention Score compared to outputs of the same age and source (52nd percentile)

Mentioned by

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3 X users
wikipedia
3 Wikipedia pages

Citations

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35 Dimensions

Readers on

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76 Mendeley
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2 CiteULike
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Title
Evolution of Substrate Specificity in a Recipient’s Enzyme Following Horizontal Gene Transfer
Published in
Molecular Biology and Evolution, June 2013
DOI 10.1093/molbev/mst115
Pubmed ID
Authors

Lianet Noda-García, Aldo R. Camacho-Zarco, Sofía Medina-Ruíz, Paul Gaytán, Mauricio Carrillo-Tripp, Vilmos Fülöp, Francisco Barona-Gómez

Abstract

Despite the prominent role of horizontal gene transfer (HGT) in shaping bacterial metabolism, little is known about the impact of HGT on the evolution of enzyme function. Specifically, what is the influence of a recently acquired gene on the function of an existing gene? For example, certain members of the genus Corynebacterium have horizontally acquired a whole l-tryptophan biosynthetic operon, whereas in certain closely related actinobacteria, for example, Mycobacterium, the trpF gene is missing. In Mycobacterium, the function of the trpF gene is performed by a dual-substrate (βα)8 phosphoribosyl isomerase (priA gene) also involved in l-histidine (hisA gene) biosynthesis. We investigated the effect of a HGT-acquired TrpF enzyme upon PriA's substrate specificity in Corynebacterium through comparative genomics and phylogenetic reconstructions. After comprehensive in vivo and enzyme kinetic analyses of selected PriA homologs, a novel (βα)8 isomerase subfamily with a specialized function in l-histidine biosynthesis, termed subHisA, was confirmed. X-ray crystallography was used to reveal active-site mutations in subHisA important for narrowing of substrate specificity, which when mutated to the naturally occurring amino acid in PriA led to gain of function. Moreover, in silico molecular dynamic analyses demonstrated that the narrowing of substrate specificity of subHisA is concomitant with loss of ancestral protein conformational states. Our results show the importance of HGT in shaping enzyme evolution and metabolism.

X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 76 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 2 3%
Netherlands 1 1%
Germany 1 1%
Canada 1 1%
Poland 1 1%
Unknown 70 92%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 24 32%
Researcher 16 21%
Student > Master 8 11%
Student > Bachelor 4 5%
Professor 4 5%
Other 11 14%
Unknown 9 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 36 47%
Biochemistry, Genetics and Molecular Biology 21 28%
Chemistry 2 3%
Computer Science 1 1%
Immunology and Microbiology 1 1%
Other 4 5%
Unknown 11 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 May 2023.
All research outputs
#6,753,656
of 25,373,627 outputs
Outputs from Molecular Biology and Evolution
#2,795
of 5,214 outputs
Outputs of similar age
#53,765
of 208,943 outputs
Outputs of similar age from Molecular Biology and Evolution
#22
of 46 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 5,214 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 17.6. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 208,943 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
We're also able to compare this research output to 46 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.