| Title |
Contribution of EmrAB efflux pumps to colistin resistance in Acinetobacter baumannii
|
|---|---|
| Published in |
Journal of Microbiology, January 2017
|
| DOI | 10.1007/s12275-017-6408-5 |
| Pubmed ID | |
| Authors |
Ming-Feng Lin, Yun-You Lin, Chung-Yu Lan |
| Abstract |
Efflux pumps play an important role in antimicrobial resistance for Acinetobacter baumannii. However, the function of the Emr pump system and the relationship between Emr and drug resistance has not been characterized in A. baumannii. In this study, four possible groups of emr-like genes were found by searching a genome database. Among them, A1S_1772 (emrB) and A1S_1773 (emrA) were demonstrated to be co-transcribed as a single operon. Moreover, during osmotic stress, A1S_1772 showed the largest change in gene expression compared to the other emrB-like genes, and deletion of A1S_1772 (AB ΔemrB) significantly slowed cell growth in 20% sucrose. Using a phenotypic microarray analysis, the AB ΔemrB mutant was more susceptible to colistin and nafcillin, paromomycin, spiramycin, and D,L-serine hydroxmate than the wild type. The spot assay, time kill assay and minimal inhibition concentration determination also indicated that the wild type could tolerate colistin better than the AB ΔemrB mutant. Finally, the increased expression levels of all emrB-like genes, including A1S_0775, A1S_0909, A1S_1772, and A1S_1799, in colistin resistance-induced A. baumannii further supported the possible involvement of the emrB genes in A. baumannii colistin resistance. Together, the Emr pump systems in A. baumannii contribute to adaptation to osmotic stress and resistance to colistin. |
Mendeley readers
The data shown below were compiled from readership statistics for 91 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 91 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 16 | 18% |
| Student > Ph. D. Student | 15 | 16% |
| Student > Bachelor | 14 | 15% |
| Student > Master | 8 | 9% |
| Student > Doctoral Student | 3 | 3% |
| Other | 4 | 4% |
| Unknown | 31 | 34% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 21 | 23% |
| Immunology and Microbiology | 13 | 14% |
| Agricultural and Biological Sciences | 8 | 9% |
| Chemistry | 5 | 5% |
| Medicine and Dentistry | 4 | 4% |
| Other | 8 | 9% |
| Unknown | 32 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 January 2017.
All research outputs
#21,476,880
of 23,975,976 outputs
Outputs from Journal of Microbiology
#677
of 842 outputs
Outputs of similar age
#361,743
of 424,899 outputs
Outputs of similar age from Journal of Microbiology
#19
of 24 outputs
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