| Title |
Molecular characterization of previously elusive badnaviruses associated with symptomatic cacao in the New World
|
|---|---|
| Published in |
Archives of Virology, January 2017
|
| DOI | 10.1007/s00705-017-3235-2 |
| Pubmed ID | |
| Authors |
Nomatter Chingandu, Muhammad Zia-ur-rehman, Thyail N. Sreenivasan, Surendra Surujdeo-Maharaj, Pathmanathan Umaharan, Osman A. Gutierrez, Judith K. Brown |
| Abstract |
Suspected virus-like symptoms were observed in cacao plants in Trinidad during 1943, and the viruses associated with these symptoms were designated as strains A and B of cacao Trinidad virus (CTV). However, viral etiology has not been demonstrated for either phenotype. Total DNA was isolated from symptomatic cacao leaves exhibiting the CTV A and B phenotypes and subjected to Illumina HiSeq and Sanger DNA sequencing. Based on de novo assembly, two apparently full-length badnavirus genomes of 7,533 and 7,454 nucleotides (nt) were associated with CTV strain A and B, respectively. The Trinidad badnaviral genomes contained four open reading frames, three of which are characteristic of other known badnaviruses, and a fourth that is present in only some badnaviruses. Both badnaviral genomes harbored hallmark caulimovirus-like features, including a tRNA(Met) priming site, a TATA box, and a polyadenylation-like signal. Pairwise comparisons of the RT-RNase H region indicated that the Trinidad isolates share 57-71% nt sequence identity with other known badnaviruses. Based on the system for badnavirus species demarcation in which viruses with less than 80% nt sequence identity in the RT-RNase gene are considered members of separate species, these isolates represent two previously unidentified badnaviruses, herein named cacao mild mosaic virus and cacao yellow vein banding virus, making them the first cacao-infecting badnaviruses identified thus far in the Western Hemisphere. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 44 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 15 | 34% |
| Student > Ph. D. Student | 7 | 16% |
| Student > Master | 4 | 9% |
| Other | 3 | 7% |
| Student > Postgraduate | 2 | 5% |
| Other | 3 | 7% |
| Unknown | 10 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 22 | 50% |
| Biochemistry, Genetics and Molecular Biology | 5 | 11% |
| Unspecified | 1 | 2% |
| Nursing and Health Professions | 1 | 2% |
| Economics, Econometrics and Finance | 1 | 2% |
| Other | 2 | 5% |
| Unknown | 12 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 January 2017.
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#18,525,776
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Outputs from Archives of Virology
#3,050
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#309,801
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Outputs of similar age from Archives of Virology
#25
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