| Title |
Chlamydomonas (Chlorophyceae) colony PCR
|
|---|---|
| Published in |
Protoplasma, February 2009
|
| DOI | 10.1007/s00709-009-0036-9 |
| Pubmed ID | |
| Authors |
Muqing Cao, Yu Fu, Yan Guo, Junmin Pan |
| Abstract |
The ease and effectiveness of colony polymerase chain reaction (PCR) has allowed rapid amplification of DNA fragments and screening of large number of colonies of interest including transformants and mutants with genetic manipulations. Here, we evaluated colony PCR in Chlamydomonas. Individual colonies were treated with 10 mM ethylenediaminetetraacetic acid (EDTA) or Chelex-100 and the resulting clear cell lysate was used for PCR reaction. Either genomic DNA or plasmid DNA incorporated into the genome was equally amplified. We found that the Chelex method is superior to EDTA method in certain cases. This colony PCR technique will bypass the tedious process of isolating genomic DNA for PCR reaction and will make it possible for rapid amplification of genomic DNA fragments as well as rapid large-scale screening of transformants. |
Mendeley readers
The data shown below were compiled from readership statistics for 188 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 2 | 1% |
| United Kingdom | 1 | <1% |
| Mexico | 1 | <1% |
| Denmark | 1 | <1% |
| Greece | 1 | <1% |
| Unknown | 182 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 45 | 24% |
| Student > Bachelor | 36 | 19% |
| Student > Master | 31 | 16% |
| Researcher | 19 | 10% |
| Student > Postgraduate | 9 | 5% |
| Other | 19 | 10% |
| Unknown | 29 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 86 | 46% |
| Biochemistry, Genetics and Molecular Biology | 50 | 27% |
| Engineering | 4 | 2% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 2% |
| Immunology and Microbiology | 3 | 2% |
| Other | 6 | 3% |
| Unknown | 36 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 January 2014.
All research outputs
#7,429,502
of 22,713,403 outputs
Outputs from Protoplasma
#132
of 968 outputs
Outputs of similar age
#32,937
of 93,802 outputs
Outputs of similar age from Protoplasma
#1
of 1 outputs
Altmetric has tracked 22,713,403 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 968 research outputs from this source. They receive a mean Attention Score of 2.4. This one has gotten more attention than average, scoring higher than 61% of its peers.
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We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them