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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Monitoring Drug Target Engagement in Cells and Tissues Using the Cellular Thermal Shift Assay
|
|---|---|
| Published in |
Science, July 2013
|
| DOI | 10.1126/science.1233606 |
| Pubmed ID | |
| Authors |
Daniel Martinez Molina, Rozbeh Jafari, Marina Ignatushchenko, Takahiro Seki, E. Andreas Larsson, Chen Dan, Lekshmy Sreekumar, Yihai Cao, Pär Nordlund |
| Abstract |
The efficacy of therapeutics is dependent on a drug binding to its cognate target. Optimization of target engagement by drugs in cells is often challenging, because drug binding cannot be monitored inside cells. We have developed a method for evaluating drug binding to target proteins in cells and tissue samples. This cellular thermal shift assay (CETSA) is based on the biophysical principle of ligand-induced thermal stabilization of target proteins. Using this assay, we validated drug binding for a set of important clinical targets and monitored processes of drug transport and activation, off-target effects and drug resistance in cancer cell lines, as well as drug distribution in tissues. CETSA is likely to become a valuable tool for the validation and optimization of drug target engagement. |
X Demographics
The data shown below were collected from the profiles of 57 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 12 | 21% |
| United Kingdom | 6 | 11% |
| Canada | 3 | 5% |
| Netherlands | 3 | 5% |
| Spain | 3 | 5% |
| Australia | 2 | 4% |
| Japan | 1 | 2% |
| Sweden | 1 | 2% |
| Paraguay | 1 | 2% |
| Other | 3 | 5% |
| Unknown | 22 | 39% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 40 | 70% |
| Scientists | 13 | 23% |
| Science communicators (journalists, bloggers, editors) | 2 | 4% |
| Practitioners (doctors, other healthcare professionals) | 2 | 4% |
Mendeley readers
The data shown below were compiled from readership statistics for 1,207 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 9 | <1% |
| United Kingdom | 7 | <1% |
| France | 5 | <1% |
| Japan | 5 | <1% |
| Netherlands | 2 | <1% |
| Germany | 2 | <1% |
| Singapore | 1 | <1% |
| Taiwan | 1 | <1% |
| Belgium | 1 | <1% |
| Other | 5 | <1% |
| Unknown | 1169 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 278 | 23% |
| Student > Ph. D. Student | 259 | 21% |
| Student > Master | 103 | 9% |
| Student > Bachelor | 94 | 8% |
| Student > Doctoral Student | 58 | 5% |
| Other | 202 | 17% |
| Unknown | 213 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 296 | 25% |
| Agricultural and Biological Sciences | 240 | 20% |
| Chemistry | 202 | 17% |
| Pharmacology, Toxicology and Pharmaceutical Science | 59 | 5% |
| Medicine and Dentistry | 53 | 4% |
| Other | 94 | 8% |
| Unknown | 263 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 158. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 February 2024.
All research outputs
#262,314
of 26,017,215 outputs
Outputs from Science
#7,246
of 83,593 outputs
Outputs of similar age
#1,685
of 209,760 outputs
Outputs of similar age from Science
#65
of 856 outputs
Altmetric has tracked 26,017,215 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 98th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 83,593 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 65.8. This one has done particularly well, scoring higher than 91% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 209,760 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 98% of its contemporaries.
We're also able to compare this research output to 856 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.