| Title |
Extracts from Black Carrot Tissue Culture as Potent Anticancer Agents
|
|---|---|
| Published in |
Plant Foods for Human Nutrition, July 2013
|
| DOI | 10.1007/s11130-013-0371-z |
| Pubmed ID | |
| Authors |
Canan Sevimli-Gur, Burcu Cetin, Seref Akay, Sultan Gulce-Iz, Ozlem Yesil-Celiktas |
| Abstract |
Black carrots contain anthocyanins possessing enhanced physiological activities. Explants of young black carrot shoots were cultured in Murashige and Skoog (MS) medium for callus initiation and were transferred to new MS medium supplemented with four different combinations of 2,4-dichlorophenoxyacetic acid and kinetin. Subsequently, the lyophilized calli and black carrot harvested from fields were subjected to ultrasound extraction with ethanol at a ratio of 1:15 (w:v). Obtained extracts were applied to various human cancer cell lines including MCF-7 SK-BR-3 and MDA-MB-231 (human breast adenocarcinomas), HT-29 (human colon adenocarcinoma), PC-3 (human prostate adenocarcinoma), Neuro 2A (Musmusculus neuroblastoma) cancer cell lines and VERO (African green monkey kidney) normal cell line by MTT assay. The highest cytotoxic activity was achieved against Neuro-2A cell lines exhibiting viability of 38-46% at 6.25 μg/ml concentration for all calli and natural extracts. However, a significantly high IC50 value of 170.13 μg/ml was attained in normal cell line VERO indicating that its natural counterpart is an ideal candidate for treatment of brain cancer without causing negative effects to normal healthy cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 64 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Turkey | 1 | 2% |
| Unknown | 63 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 13% |
| Student > Bachelor | 8 | 13% |
| Student > Master | 8 | 13% |
| Researcher | 7 | 11% |
| Other | 5 | 8% |
| Other | 10 | 16% |
| Unknown | 18 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 10 | 16% |
| Engineering | 6 | 9% |
| Biochemistry, Genetics and Molecular Biology | 5 | 8% |
| Medicine and Dentistry | 5 | 8% |
| Chemistry | 5 | 8% |
| Other | 7 | 11% |
| Unknown | 26 | 41% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 November 2015.
All research outputs
#7,429,502
of 22,713,403 outputs
Outputs from Plant Foods for Human Nutrition
#255
of 701 outputs
Outputs of similar age
#65,005
of 194,389 outputs
Outputs of similar age from Plant Foods for Human Nutrition
#6
of 8 outputs
Altmetric has tracked 22,713,403 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 701 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.3. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 194,389 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.
We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than 2 of them.