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Braf Mutations Initiate the Development of Rat Gliomas Induced by Postnatal Exposure to N-Ethyl-N-Nitrosourea

Overview of attention for article published in American Journal of Pathology, October 2016
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Title
Braf Mutations Initiate the Development of Rat Gliomas Induced by Postnatal Exposure to N-Ethyl-N-Nitrosourea
Published in
American Journal of Pathology, October 2016
DOI 10.1016/j.ajpath.2016.05.024
Pubmed ID
Authors

Qi Wang, Kaishi Satomi, Ji Eun Oh, Barbara Hutter, Benedikt Brors, Nicolle Diessl, Hai-Kun Liu, Stephan Wolf, Otmar Wiestler, Paul Kleihues, Bernd Koelsch, Andrea Kindler-Röhrborn, Hiroko Ohgaki

Abstract

A single dose of N-ethyl-N-nitrosourea (ENU) during late prenatal or early postnatal development induces a high incidence of malignant schwannomas and gliomas in rats. Although T->A mutations in the transmembrane domain of the Neu (c-ErbB-2) gene are the driver mutations in ENU-induced malignant schwannomas, the molecular basis of ENU-induced gliomas remains enigmatic. We performed whole-genome sequencing of gliomas that developed in three BDIV and two BDIX rats exposed to a single dose of 80 mg ENU/kg body weight on postnatal day one. T:A->A:T and T:A->C:G mutations, which are typical for ENU-induced mutagenesis, were predominant (41% to 55% of all somatic single nucleotide mutations). T->A mutations were identified in all five rat gliomas at Braf codon 545 (V545E), which corresponds to the human BRAF V600E. Additional screening revealed that 33 gliomas in BDIV rats and 12 gliomas in BDIX rats all carried a Braf V545E mutation, whereas peritumoral brain tissue of either strain had the wild-type sequence. The gliomas were immunoreactive to BRAF V600E antibody. These results indicate that Braf mutation is a frequent early event in the development of rat gliomas caused by a single dose of ENU.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Other 2 10%
Student > Master 2 10%
Researcher 2 10%
Student > Ph. D. Student 2 10%
Student > Bachelor 1 5%
Other 2 10%
Unknown 10 48%
Readers by discipline Count As %
Medicine and Dentistry 5 24%
Biochemistry, Genetics and Molecular Biology 2 10%
Agricultural and Biological Sciences 1 5%
Veterinary Science and Veterinary Medicine 1 5%
Neuroscience 1 5%
Other 1 5%
Unknown 10 48%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 October 2017.
All research outputs
#20,817,194
of 25,576,801 outputs
Outputs from American Journal of Pathology
#5,221
of 5,928 outputs
Outputs of similar age
#258,074
of 333,150 outputs
Outputs of similar age from American Journal of Pathology
#47
of 59 outputs
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