Title |
Immunization with Apical Membrane Antigen 1 Confers Sterile Infection-Blocking Immunity against Plasmodium Sporozoite Challenge in a Rodent Model
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Published in |
Infection and Immunity, July 2013
|
DOI | 10.1128/iai.00544-13 |
Pubmed ID | |
Authors |
Sophie Schussek, Angela Trieu, Simon H. Apte, John Sidney, Alessandro Sette, Denise L. Doolan |
Abstract |
Apical membrane antigen 1 (AMA-1) is a leading blood-stage malaria vaccine candidate. Consistent with a key role in erythrocytic invasion, AMA-1-specific antibodies have been implicated in AMA-1-induced protective immunity. AMA-1 is also expressed in sporozoites and in mature liver schizonts where it may be a target of protective cell-mediated immunity. Here, we demonstrate for the first time that immunization with AMA-1 can induce sterile infection-blocking immunity against Plasmodium sporozoite challenge in 80% of immunized mice. Significantly higher levels of gamma interferon (IFN-γ)/interleukin-2 (IL-2)/tumor necrosis factor (TNF) multifunctional T cells were noted in immunized mice than in control mice. We also report the first identification of minimal CD8(+) and CD4(+) T cell epitopes on Plasmodium yoelii AMA-1. These data establish AMA-1 as a target of both preerythrocytic- and erythrocytic-stage protective immune responses and validate vaccine approaches designed to induce both cellular and humoral immunity. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 3% |
Unknown | 34 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 10 | 29% |
Student > Ph. D. Student | 6 | 17% |
Other | 5 | 14% |
Student > Bachelor | 3 | 9% |
Student > Master | 2 | 6% |
Other | 4 | 11% |
Unknown | 5 | 14% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 12 | 34% |
Agricultural and Biological Sciences | 10 | 29% |
Biochemistry, Genetics and Molecular Biology | 4 | 11% |
Medicine and Dentistry | 3 | 9% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
Other | 0 | 0% |
Unknown | 5 | 14% |