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Mendeley readers
Attention Score in Context
| Title |
Detection of West Nile Virus Antigen in Mosquitoes and Avian Tissues by a Monoclonal Antibody-Based Capture Enzyme Immunoassay
|
|---|---|
| Published in |
Journal of Clinical Microbiology, June 2002
|
| DOI | 10.1128/jcm.40.6.2023-2030.2002 |
| Pubmed ID | |
| Authors |
Ann R. Hunt, Roy A. Hall, Amy J. Kerst, Roger S. Nasci, Harry M. Savage, Nicholas A. Panella, Kristy L. Gottfried, Kristen L. Burkhalter, John T. Roehrig |
| Abstract |
An antigen capture immunoassay to detect West Nile (WN) virus antigen in infected mosquitoes and avian tissues has been developed. With this assay purified WN virus was detected at a concentration of 32 pg/0.1 ml, and antigen in infected suckling mouse brain and laboratory-infected mosquito pools could be detected when the WN virus titer was 10(2.1) to 10(3.7) PFU/0.1 ml. In a blindly coded set of field-collected mosquito pools (n = 100), this assay detected WN virus antigen in 12 of 18 (66.7%) TaqMan-positive pools, whereas traditional reverse transcriptase PCR detected 10 of 18 (55.5%) positive pools. A sample set of 73 organ homogenates from naturally infected American crows was also examined by WN virus antigen capture immunoassay and TaqMan for the presence of WN virus. The antigen capture assay detected antigen in 30 of 34 (88.2%) TaqMan-positive tissues. Based upon a TaqMan-generated standard curve of infectious WN virus, the limit of detection in the antigen capture assay for avian tissue homogenates was approximately 10(3) PFU/0.1 ml. The recommended WN virus antigen capture protocol, which includes a capture assay followed by a confirmatory inhibition assay used to retest presumptive positive samples, could distinguish between the closely related WN and St. Louis encephalitis viruses in virus-infected mosquito pools and avian tissues. Therefore, this immunoassay demonstrates adequate sensitivity and specificity for surveillance of WN virus activity in mosquito vectors and avian hosts, and, in addition, it is easy to perform and relatively inexpensive compared with the TaqMan assay. |
Mendeley readers
The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 3% |
| Australia | 1 | 3% |
| Unknown | 32 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 18% |
| Student > Ph. D. Student | 6 | 18% |
| Student > Master | 4 | 12% |
| Other | 4 | 12% |
| Student > Doctoral Student | 3 | 9% |
| Other | 6 | 18% |
| Unknown | 5 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 16 | 47% |
| Veterinary Science and Veterinary Medicine | 5 | 15% |
| Medicine and Dentistry | 3 | 9% |
| Environmental Science | 1 | 3% |
| Nursing and Health Professions | 1 | 3% |
| Other | 3 | 9% |
| Unknown | 5 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 August 2010.
All research outputs
#4,736,199
of 22,952,268 outputs
Outputs from Journal of Clinical Microbiology
#2,912
of 13,488 outputs
Outputs of similar age
#16,239
of 120,446 outputs
Outputs of similar age from Journal of Clinical Microbiology
#14
of 111 outputs
Altmetric has tracked 22,952,268 research outputs across all sources so far. Compared to these this one has done well and is in the 76th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 13,488 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.5. This one has gotten more attention than average, scoring higher than 66% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 120,446 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 111 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 77% of its contemporaries.