Title |
Assessing the Fecal Microbiota: An Optimized Ion Torrent 16S rRNA Gene-Based Analysis Protocol
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Published in |
PLOS ONE, July 2013
|
DOI | 10.1371/journal.pone.0068739 |
Pubmed ID | |
Authors |
Christian Milani, Arancha Hevia, Elena Foroni, Sabrina Duranti, Francesca Turroni, Gabriele Andrea Lugli, Borja Sanchez, Rebeca Martín, Miguel Gueimonde, Douwe van Sinderen, Abelardo Margolles, Marco Ventura |
Abstract |
Assessing the distribution of 16S rRNA gene sequences within a biological sample represents the current state-of-the-art for determination of human gut microbiota composition. Advances in dissecting the microbial biodiversity of this ecosystem have very much been dependent on the development of novel high-throughput DNA sequencing technologies, like the Ion Torrent. However, the precise representation of this bacterial community may be affected by the protocols used for DNA extraction as well as by the PCR primers employed in the amplification reaction. Here, we describe an optimized protocol for 16S rRNA gene-based profiling of the fecal microbiota. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 17% |
France | 1 | 8% |
Canada | 1 | 8% |
Norway | 1 | 8% |
Belgium | 1 | 8% |
Unknown | 6 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 7 | 58% |
Scientists | 5 | 42% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 5 | 1% |
Brazil | 4 | 1% |
United Kingdom | 3 | <1% |
France | 2 | <1% |
Uruguay | 2 | <1% |
South Africa | 2 | <1% |
Canada | 2 | <1% |
Chile | 1 | <1% |
New Zealand | 1 | <1% |
Other | 3 | <1% |
Unknown | 364 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 88 | 23% |
Researcher | 83 | 21% |
Student > Master | 44 | 11% |
Student > Bachelor | 32 | 8% |
Student > Postgraduate | 19 | 5% |
Other | 74 | 19% |
Unknown | 49 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 160 | 41% |
Biochemistry, Genetics and Molecular Biology | 54 | 14% |
Medicine and Dentistry | 34 | 9% |
Immunology and Microbiology | 33 | 8% |
Environmental Science | 12 | 3% |
Other | 32 | 8% |
Unknown | 64 | 16% |