| Title |
Development of a PCR technique specific for Demodex injai in biological specimens
|
|---|---|
| Published in |
Parasitology Research, July 2013
|
| DOI | 10.1007/s00436-013-3531-z |
| Pubmed ID | |
| Authors |
N. Sastre, I. Ravera, D. Ferreira, L. Altet, A. Sánchez, M. Bardagí, O. Francino, L. Ferrer |
| Abstract |
The identification of Demodex injai as a second Demodex species of dog opened new questions and challenges in the understanding on the Demodex-host relationships. In this paper, we describe the development of a conventional PCR technique based on published genome sequences of D. injai from GenBank that specifically detects DNA from D. injai. This technique amplifies a 238-bp fragment corresponding to a region of the mitochondrial 16S rDNA of D. injai. The PCR was positive in DNA samples obtained from mites identified morphologically as D. injai, which served as positive controls, as well as in samples from three cases of demodicosis associated with proliferation of mites identified as D. injai. Furthermore, the PCR was positive in 2 out of 19 healthy dogs. Samples of Demodex canis and Demodex folliculorum were consistently negative. Skin samples from seven dogs with generalized demodicosis caused by D. canis were all negative in the D. injai-specific PCR, demonstrating that in generalized canine demodicosis, mite proliferation is species-specific. This technique can be a useful tool in the diagnosis and in epidemiologic and pathogenic studies. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Czechia | 1 | 4% |
| Unknown | 22 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 17% |
| Other | 3 | 13% |
| Researcher | 3 | 13% |
| Student > Doctoral Student | 2 | 9% |
| Student > Postgraduate | 2 | 9% |
| Other | 3 | 13% |
| Unknown | 6 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 7 | 30% |
| Agricultural and Biological Sciences | 3 | 13% |
| Medicine and Dentistry | 3 | 13% |
| Biochemistry, Genetics and Molecular Biology | 2 | 9% |
| Unspecified | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 6 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 July 2013.
All research outputs
#20,196,270
of 22,714,025 outputs
Outputs from Parasitology Research
#2,869
of 3,775 outputs
Outputs of similar age
#170,619
of 194,446 outputs
Outputs of similar age from Parasitology Research
#39
of 59 outputs
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