| Title |
Dirofilaria repens microfilariae in Aedes vexans mosquitoes in Slovakia
|
|---|---|
| Published in |
Parasitology Research, July 2013
|
| DOI | 10.1007/s00436-013-3526-9 |
| Pubmed ID | |
| Authors |
Eva Bocková, Ivo Rudolf, Alica Kočišová, Lenka Betášová, Kristýna Venclíková, Jan Mendel, Zdeněk Hubálek |
| Abstract |
In this study, we screened field-caught mosquitoes for presence of Dirofilaria spp. by using a polymerase chain reaction (PCR) assay. Potential occurrence of Dirofilaria repens and Dirofilaria immitis microfilariae was examined in 3,600 mosquitoes of eight species (Aedes vexans, Aedes cinereus, Aedes rossicus, Culex pipiens, Culiseta annulata, Ochlerotatus sticticus, Ochlerotatus cantans and Ochlerotatus caspius) collected from five locations in two districts (Kosice and Trebisov) of Eastern Slovakia, endemic region of canine dirofilariasis. Collection of mosquitoes was performed between May and August 2012 in premises known to be inhabited by Dirofilaria-infected dogs. PCR assays were performed on 72 pools, each pool containing 50 mosquitoes of the same species, collected on the same location. Each pool was examined separately for the presence of D. immitis and D. repens, respectively. A positive finding of D. repens was recorded in one pool of A. vexans mosquitoes collected in Košické Olšany village. Minimum infection rate in A. vexans was 1:1,750, i.e. 0.57 per 1,000 mosquitoes. The identity of D. repens was confirmed by direct sequencing of PCR product which has shown 100% homology with sequence attributed to D. repens (GenBank accession number AJ271614). This study represents the first molecular evidence of D. repens microfilariae in mosquitoes in Slovakia and highlights a need for better surveillance of zoonotic dirofilariasis in central Europe. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 51 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Slovakia | 1 | 2% |
| Unknown | 50 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 20% |
| Student > Ph. D. Student | 6 | 12% |
| Student > Master | 5 | 10% |
| Student > Doctoral Student | 4 | 8% |
| Student > Postgraduate | 3 | 6% |
| Other | 9 | 18% |
| Unknown | 14 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 14 | 27% |
| Veterinary Science and Veterinary Medicine | 7 | 14% |
| Medicine and Dentistry | 5 | 10% |
| Biochemistry, Genetics and Molecular Biology | 4 | 8% |
| Immunology and Microbiology | 3 | 6% |
| Other | 4 | 8% |
| Unknown | 14 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 April 2014.
All research outputs
#6,764,961
of 22,714,025 outputs
Outputs from Parasitology Research
#504
of 3,775 outputs
Outputs of similar age
#57,474
of 194,569 outputs
Outputs of similar age from Parasitology Research
#8
of 56 outputs
Altmetric has tracked 22,714,025 research outputs across all sources so far. This one has received more attention than most of these and is in the 69th percentile.
So far Altmetric has tracked 3,775 research outputs from this source. They receive a mean Attention Score of 2.7. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 194,569 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 56 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 85% of its contemporaries.