| Title |
Characterization and application of endogenous phase-dependent promoters in Bacillus subtilis
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, February 2017
|
| DOI | 10.1007/s00253-017-8142-7 |
| Pubmed ID | |
| Authors |
Sen Yang, Guocheng Du, Jian Chen, Zhen Kang |
| Abstract |
Bacillus subtilis as an important host has been widely used in synthetic biology, metabolic engineering, and production of industrial enzymes. To fully take advantage of this organism, 114 endogenous putative promoters were measured with a green fluorescent protein reporter and four classes of phase-dependent promoters (class I: exponential phase; class II: middle-log and early stationary phases; class III: lag-log and stationary phases; class IV: stationary phase) with different strengths were identified. The transcriptional strengths ranged from 0.03 to 2.03-fold of that of the commonly used strong promoter P43. On this basis, the temperature (for instance P bltD , P ydaD , and P gerBC ) and pH (such as P abrB , P ydjO , and P opuE ) inducible phase-dependent promoters were further identified and characterized. In comparison, P abrB (class I), P spoVG (class II), and P lytR (class III) achieved the highest expression levels of esterase, keratinase, and alkaline polygalacturonate lyase, respectively. The constructed phase-dependent promoter library should have great application potentials for enzyme production, metabolic engineering, and synthetic biology. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 89 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| China | 1 | 1% |
| Unknown | 88 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 20 | 22% |
| Student > Master | 13 | 15% |
| Researcher | 10 | 11% |
| Student > Bachelor | 7 | 8% |
| Student > Doctoral Student | 4 | 4% |
| Other | 6 | 7% |
| Unknown | 29 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 37 | 42% |
| Agricultural and Biological Sciences | 15 | 17% |
| Chemical Engineering | 2 | 2% |
| Engineering | 2 | 2% |
| Energy | 1 | 1% |
| Other | 1 | 1% |
| Unknown | 31 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 December 2019.
All research outputs
#7,764,167
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#2,616
of 8,034 outputs
Outputs of similar age
#145,217
of 435,346 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#23
of 101 outputs
Altmetric has tracked 24,119,703 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 8,034 research outputs from this source. They receive a mean Attention Score of 4.3. This one has gotten more attention than average, scoring higher than 65% of its peers.
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We're also able to compare this research output to 101 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 76% of its contemporaries.