| Title |
Electrolyzed-reduced water protects against oxidative damage to DNA, RNA, and protein
|
|---|---|
| Published in |
Applied Biochemistry and Biotechnology, January 2018
|
| DOI | 10.1385/abab:135:2:133 |
| Pubmed ID | |
| Authors |
Mi Young Lee, Yoon Kyoung Kim, Kun Kul Ryoo, Yoon Bae Lee, Eun Ju Park |
| Abstract |
The generation of reactive oxygen species is thought to cause extensive oxidative damage to various biomolecules such as DNA, RNA, and protein. In this study, the preventive, suppressive, and protective effects of in vitro supplementation with electrolyzed-reduced water on H2O2-induced DNA damage in human lymphocytes were examined using a comet assay. Pretreatment, cotreatment, and posttreatment with electrolyzed-reduced water enhanced human lymphocyte resistance to the DNA strand breaks induced by H2O2 in vitro. Moreover, electrolyzed-reduced water was much more effective than diethylpyrocarbonate-treated water in preventing total RNA degradation at 4 and 25 degrees C. In addition, electrolyzed-reduced water completely prevented the oxidative cleavage of horseradish peroxidase, as determined using sodium dodecyl sulfate-polyacrylamide gels. Enhancement of the antioxidant activity of ascorbic acid dissolved in electrolyzed-reduced water was about threefold that of ascorbic acid dissolved in nonelectrolyzed deionized water, as measured by a xanthine-xanthine oxidase superoxide scavenging assay system, suggesting an inhibitory effect of electrolyzedreduced water on the oxidation of ascorbic acid. |
X Demographics
The data shown below were collected from the profiles of 27 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 17 | 63% |
| Jordan | 1 | 4% |
| Singapore | 1 | 4% |
| Spain | 1 | 4% |
| Unknown | 7 | 26% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 26 | 96% |
| Unknown | 1 | 4% |
Mendeley readers
The data shown below were compiled from readership statistics for 50 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 2% |
| Mexico | 1 | 2% |
| Unknown | 48 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 18% |
| Researcher | 8 | 16% |
| Student > Master | 7 | 14% |
| Student > Bachelor | 6 | 12% |
| Student > Doctoral Student | 4 | 8% |
| Other | 8 | 16% |
| Unknown | 8 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 14 | 28% |
| Medicine and Dentistry | 5 | 10% |
| Chemistry | 5 | 10% |
| Engineering | 3 | 6% |
| Business, Management and Accounting | 2 | 4% |
| Other | 8 | 16% |
| Unknown | 13 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 35. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 November 2023.
All research outputs
#1,178,479
of 25,758,695 outputs
Outputs from Applied Biochemistry and Biotechnology
#6
of 2,780 outputs
Outputs of similar age
#26,850
of 453,687 outputs
Outputs of similar age from Applied Biochemistry and Biotechnology
#1
of 20 outputs
Altmetric has tracked 25,758,695 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 95th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,780 research outputs from this source. They receive a mean Attention Score of 3.1. This one has done particularly well, scoring higher than 99% of its peers.
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We're also able to compare this research output to 20 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.