| Title |
RNAi-Mediated Simultaneous Resistance Against Three RNA Viruses in Potato
|
|---|---|
| Published in |
Molecular Biotechnology, February 2017
|
| DOI | 10.1007/s12033-017-9995-9 |
| Pubmed ID | |
| Authors |
Amir Hameed, Muhammad Nouman Tahir, Shaheen Asad, Rakhshanda Bilal, Joyce Van Eck, Georg Jander, Shahid Mansoor |
| Abstract |
RNA interference (RNAi) technology has been successfully applied in stacking resistance against viruses in numerous crop plants. During RNAi, the production of small interfering RNAs (siRNAs) from template double-standard RNA (dsRNA) derived from expression constructs provides an on-switch for triggering homology-based targeting of cognate viral transcripts, hence generating a pre-programmed immunity in transgenic plants prior to virus infection. In the current study, transgenic potato lines (Solanum tuberosum cv. Desiree) were generated, expressing fused viral coat protein coding sequences from Potato virus X (PVX), Potato virus Y (PVY), and Potato virus S (PVS) as a 600-bp inverted repeat expressed from a constitutive 35S promoter. The expression cassette (designated Ec1/p5941) was designed to generate dsRNAs having a hairpin loop configuration. The transgene insertions were confirmed by glufosinate resistance, gene-specific PCR, and Southern blotting. Regenerated lines were further assayed for resistance to virus inoculation for up to two consecutive crop seasons. Nearly 100% resistance against PVX, PVY, and PVS infection was observed in transgenic lines when compared with untransformed controls, which developed severe viral disease symptoms. These results establish the efficacy of RNAi using the coat protein gene as a potential target for the successful induction of stable antiviral immunity in potatoes. |
X Demographics
The data shown below were collected from the profiles of 11 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | 36% |
| Australia | 1 | 9% |
| United Kingdom | 1 | 9% |
| Pakistan | 1 | 9% |
| Canada | 1 | 9% |
| Unknown | 3 | 27% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 7 | 64% |
| Scientists | 4 | 36% |
Mendeley readers
The data shown below were compiled from readership statistics for 61 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 61 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 12 | 20% |
| Student > Ph. D. Student | 7 | 11% |
| Student > Bachelor | 5 | 8% |
| Student > Master | 5 | 8% |
| Other | 4 | 7% |
| Other | 7 | 11% |
| Unknown | 21 | 34% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 21 | 34% |
| Biochemistry, Genetics and Molecular Biology | 14 | 23% |
| Medicine and Dentistry | 2 | 3% |
| Unspecified | 1 | 2% |
| Arts and Humanities | 1 | 2% |
| Other | 2 | 3% |
| Unknown | 20 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 August 2017.
All research outputs
#4,613,141
of 22,953,506 outputs
Outputs from Molecular Biotechnology
#113
of 970 outputs
Outputs of similar age
#98,630
of 426,820 outputs
Outputs of similar age from Molecular Biotechnology
#1
of 2 outputs
Altmetric has tracked 22,953,506 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 970 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done well, scoring higher than 88% of its peers.
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