| Title |
Large-Scale Production of Phospholipase D from Streptomyces racemochromogenes and Its Application to Soybean Lecithin Modification
|
|---|---|
| Published in |
Applied Biochemistry and Biotechnology, September 2011
|
| DOI | 10.1007/s12010-011-9370-4 |
| Pubmed ID | |
| Authors |
Yozo Nakazawa, Yoshimasa Sagane, Shin-ichiro Sakurai, Masataka Uchino, Hiroaki Sato, Kazuki Toeda, Katsumi Takano |
| Abstract |
Phospholipase D (PLD) catalyzes transphosphatidylation, causing inter-conversion of the polar head group of phospholipids and phospholipid hydrolysis. Previously, we cloned PLD103, a PLD with high transphosphatidylation activity, from Streptomyces racemochromogenes strain 10-3. Here, we report the construction of an expression system for the PLD103 gene using Streptomyces lividans as the host bacterium to achieve large-scale production. The phosphatidylcholine (PC) hydrolysis activity of S. lividans transformed with the expression plasmid containing the PLD103 gene was approximately 90-fold higher than that of the original strain. The recombinant PLD103 (rPLD103) found in the supernatant of the transformant culture medium was close to homogeneous. The rPLD103 was indistinguishable from the native enzyme in molecular mass and enzymatic properties. Additionally, rPLD103 had high transphosphatidylation activity on PC as a substrate in a simple aqueous one-phase reaction system and was able to modify the phospholipid content of soybean lecithin. Consequently, the expression system produces a stable supply of PLD, which can then be used in the production of phosphatidyl derivatives from lecithin. |
Mendeley readers
The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 33 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 6 | 18% |
| Student > Ph. D. Student | 6 | 18% |
| Student > Bachelor | 4 | 12% |
| Professor | 3 | 9% |
| Other | 2 | 6% |
| Other | 5 | 15% |
| Unknown | 7 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 39% |
| Biochemistry, Genetics and Molecular Biology | 3 | 9% |
| Chemistry | 3 | 9% |
| Immunology and Microbiology | 2 | 6% |
| Computer Science | 2 | 6% |
| Other | 3 | 9% |
| Unknown | 7 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 March 2016.
All research outputs
#7,186,090
of 22,715,151 outputs
Outputs from Applied Biochemistry and Biotechnology
#508
of 2,496 outputs
Outputs of similar age
#43,014
of 130,663 outputs
Outputs of similar age from Applied Biochemistry and Biotechnology
#4
of 12 outputs
Altmetric has tracked 22,715,151 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 2,496 research outputs from this source. They receive a mean Attention Score of 3.2. This one has done well, scoring higher than 78% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 130,663 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.
We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.