| Title |
Signal Enhancement in the HPLC-ESI-MS/MS analysis of spironolactone and its metabolites using HFIP and NH4F as eluent additives
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, February 2017
|
| DOI | 10.1007/s00216-017-0255-4 |
| Pubmed ID | |
| Authors |
Kalev Takkis, Rudolf Aro, Lenne-Triin Kõrgvee, Heili Varendi, Jana Lass, Koit Herodes, Karin Kipper |
| Abstract |
This paper describes an LC-MS/MS method to determine the concentration of spironolactone and its metabolites 7-alpha-methylthiospironolactone and canrenone in blood plasma samples. The resulting assay is simple (using protein precipitation for sample preparation) and sensitive (the lower limit of quantification is close to 0.5 ng/ml) while requiring only 50 μl of plasma, making it especially suitable for analyzing samples obtained from pediatric and neonatal patients where sample sizes are limited. The sensitivity is achieved by using ammonium fluoride as an eluent additive, which in our case amplifies the signal from our analytes in the plasma solution on average about 70 times. The method is fully validated according to the European Medicines Agency's guideline and used for the measurement of pediatric patients' samples in clinical trials for evaluating oral spironolactone's and its metabolites' pharmacokinetics in children up to 2 years of age. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 17% |
| India | 1 | 17% |
| Mexico | 1 | 17% |
| Germany | 1 | 17% |
| Unknown | 2 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 4 | 67% |
| Science communicators (journalists, bloggers, editors) | 1 | 17% |
| Members of the public | 1 | 17% |
Mendeley readers
The data shown below were compiled from readership statistics for 48 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 48 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 9 | 19% |
| Student > Ph. D. Student | 8 | 17% |
| Researcher | 5 | 10% |
| Professor > Associate Professor | 3 | 6% |
| Student > Doctoral Student | 2 | 4% |
| Other | 5 | 10% |
| Unknown | 16 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 14 | 29% |
| Pharmacology, Toxicology and Pharmaceutical Science | 5 | 10% |
| Biochemistry, Genetics and Molecular Biology | 4 | 8% |
| Medicine and Dentistry | 2 | 4% |
| Business, Management and Accounting | 1 | 2% |
| Other | 2 | 4% |
| Unknown | 20 | 42% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 April 2017.
All research outputs
#14,735,876
of 25,593,129 outputs
Outputs from Analytical & Bioanalytical Chemistry
#4,388
of 9,674 outputs
Outputs of similar age
#164,999
of 324,480 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#30
of 165 outputs
Altmetric has tracked 25,593,129 research outputs across all sources so far. This one is in the 41st percentile – i.e., 41% of other outputs scored the same or lower than it.
So far Altmetric has tracked 9,674 research outputs from this source. They receive a mean Attention Score of 3.1. This one has gotten more attention than average, scoring higher than 54% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 324,480 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 165 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 81% of its contemporaries.