| Title |
Progesterone requires heat shock protein 90 (HSP90) in human sperm to regulate motility and acrosome reaction
|
|---|---|
| Published in |
Journal of Assisted Reproduction and Genetics, February 2017
|
| DOI | 10.1007/s10815-017-0879-5 |
| Pubmed ID | |
| Authors |
Vrushali Sagare-Patil, Rashmi Bhilawadikar, Mosami Galvankar, Kusum Zaveri, Indira Hinduja, Deepak Modi |
| Abstract |
The aims of this paper were to study whether heat shock protein 90 (HSP90) is a regulator of sperm functions and to determine its association with oligoasthenozoospermia. The levels of HSP90 in sperm lysates were measured by ELISA. Localization of HSP90 and its isoforms was evaluated by immunofluorescence. Sperm motility and kinetics were assessed by computer-assisted sperm analysis. Acrosome reaction was determined by lectin staining. The levels of HSP90 were lower in oligoasthenozoospermic men and correlated positively with the number of motile spermatozoa. In capacitated human spermatozoa, HSP90α was mostly found in residual nuclear envelope, and the HSP90β isoform was higher in the flagella. Inhibition of HSP90 by geldanamycin or 17-AAG did not affect basal motility, but suppressed progesterone-mediated forward progressive motility, hyperactivation and acrosome reaction. Progesterone treatment dephosphorylated both HSP90α and HSP90β at Ser/Thr-Pro residues, but not Tyr residues. HSP90 levels are downregulated in oligoasthenozoospermia, and its functional inhibition attenuates progesterone-mediated sperm motility and acrosome reaction. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 3 | 75% |
| Unknown | 1 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Scientists | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 35 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 17% |
| Student > Ph. D. Student | 5 | 14% |
| Professor | 4 | 11% |
| Student > Bachelor | 3 | 9% |
| Lecturer | 3 | 9% |
| Other | 7 | 20% |
| Unknown | 7 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 29% |
| Veterinary Science and Veterinary Medicine | 4 | 11% |
| Medicine and Dentistry | 4 | 11% |
| Agricultural and Biological Sciences | 3 | 9% |
| Neuroscience | 2 | 6% |
| Other | 1 | 3% |
| Unknown | 11 | 31% |
Attention Score in Context
This research output has an Altmetric Attention Score of 14. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 March 2018.
All research outputs
#2,317,559
of 24,119,703 outputs
Outputs from Journal of Assisted Reproduction and Genetics
#101
of 1,697 outputs
Outputs of similar age
#44,332
of 315,266 outputs
Outputs of similar age from Journal of Assisted Reproduction and Genetics
#2
of 18 outputs
Altmetric has tracked 24,119,703 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,697 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.8. This one has done particularly well, scoring higher than 93% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 315,266 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 85% of its contemporaries.
We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 88% of its contemporaries.