| Title |
On the use of ultracentrifugal devices for routine sample preparation in biomolecular magic-angle-spinning NMR
|
|---|---|
| Published in |
Journal of Biomolecular NMR, February 2017
|
| DOI | 10.1007/s10858-017-0089-6 |
| Pubmed ID | |
| Authors |
Abhishek Mandal, Jennifer C. Boatz, Travis B. Wheeler, Patrick C. A. van der Wel |
| Abstract |
A number of recent advances in the field of magic-angle-spinning (MAS) solid-state NMR have enabled its application to a range of biological systems of ever increasing complexity. To retain biological relevance, these samples are increasingly studied in a hydrated state. At the same time, experimental feasibility requires the sample preparation process to attain a high sample concentration within the final MAS rotor. We discuss these considerations, and how they have led to a number of different approaches to MAS NMR sample preparation. We describe our experience of how custom-made (or commercially available) ultracentrifugal devices can facilitate a simple, fast and reliable sample preparation process. A number of groups have since adopted such tools, in some cases to prepare samples for sedimentation-style MAS NMR experiments. Here we argue for a more widespread adoption of their use for routine MAS NMR sample preparation. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 25% |
| Netherlands | 1 | 25% |
| Unknown | 2 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 50% |
| Members of the public | 2 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 53 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 53 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 16 | 30% |
| Student > Ph. D. Student | 10 | 19% |
| Student > Bachelor | 4 | 8% |
| Other | 4 | 8% |
| Professor > Associate Professor | 4 | 8% |
| Other | 6 | 11% |
| Unknown | 9 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 18 | 34% |
| Biochemistry, Genetics and Molecular Biology | 10 | 19% |
| Agricultural and Biological Sciences | 5 | 9% |
| Unspecified | 3 | 6% |
| Physics and Astronomy | 3 | 6% |
| Other | 2 | 4% |
| Unknown | 12 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 April 2020.
All research outputs
#3,325,874
of 23,577,654 outputs
Outputs from Journal of Biomolecular NMR
#36
of 619 outputs
Outputs of similar age
#62,126
of 312,460 outputs
Outputs of similar age from Journal of Biomolecular NMR
#1
of 9 outputs
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