High resolution solid-state NMR spectroscopy of the Yersinia pestis outer membrane protein Ail in lipid membranes

Yong Yao, Samit Kumar Dutta, Sang Ho Park, Ratan Rai, L. Miya Fujimoto, Andrey A. Bobkov, Stanley J. Opella, Francesca M. Marassi

The outer membrane protein Ail (Adhesion invasion locus) is one of the most abundant proteins on the cell surface of Yersinia pestis during human infection. Its functions are expressed through interactions with a variety of human host proteins, and are essential for microbial virulence. Structures of Ail have been determined by X-ray diffraction and solution NMR spectroscopy, but those samples contained detergents that interfere with functionality, thus, precluding analysis of the structural basis for Ail's biological activity. Here, we demonstrate that high-resolution solid-state NMR spectra can be obtained from samples of Ail in detergent-free phospholipid liposomes, prepared with a lipid to protein molar ratio of 100. The spectra, obtained with (13)C or (1)H detection, have very narrow line widths (0.40-0.60 ppm for (13)C, 0.11-0.15 ppm for (1)H, and 0.46-0.64 ppm for (15)N) that are consistent with a high level of sample homogeneity. The spectra enable resonance assignments to be obtained for N, CO, CA and CB atomic sites from 75 out of 156 residues in the sequence of Ail, including 80% of the transmembrane region. The (1)H-detected solid-state NMR (1)H/(15)N correlation spectra obtained for Ail in liposomes compare very favorably with the solution NMR (1)H/(15)N TROSY spectra obtained for Ail in nanodiscs prepared with a similar lipid to protein molar ratio. These results set the stage for studies of the molecular basis of the functional interactions of Ail with its protein partners from human host cells, as well as the development of drugs targeting Ail.