| Title |
PCR-based detection of gene transfer vectors: application to gene doping surveillance
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, August 2013
|
| DOI | 10.1007/s00216-013-7264-8 |
| Pubmed ID | |
| Authors |
Irene C. Perez, Caroline Le Guiner, Weiyi Ni, Jennifer Lyles, Philippe Moullier, Richard O. Snyder |
| Abstract |
Athletes who illicitly use drugs to enhance their athletic performance are at risk of being banned from sports competitions. Consequently, some athletes may seek new doping methods that they expect to be capable of circumventing detection. With advances in gene transfer vector design and therapeutic gene transfer, and demonstrations of safety and therapeutic benefit in humans, there is an increased probability of the pursuit of gene doping by athletes. In anticipation of the potential for gene doping, assays have been established to directly detect complementary DNA of genes that are top candidates for use in doping, as well as vector control elements. The development of molecular assays that are capable of exposing gene doping in sports can serve as a deterrent and may also identify athletes who have illicitly used gene transfer for performance enhancement. PCR-based methods to detect foreign DNA with high reliability, sensitivity, and specificity include TaqMan real-time PCR, nested PCR, and internal threshold control PCR. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 4 | 67% |
| United States | 1 | 17% |
| Grenada | 1 | 17% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 50% |
| Scientists | 2 | 33% |
| Practitioners (doctors, other healthcare professionals) | 1 | 17% |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 3% |
| Ireland | 1 | 3% |
| Unknown | 35 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 7 | 19% |
| Student > Bachelor | 6 | 16% |
| Student > Ph. D. Student | 6 | 16% |
| Researcher | 4 | 11% |
| Lecturer | 3 | 8% |
| Other | 4 | 11% |
| Unknown | 7 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 24% |
| Agricultural and Biological Sciences | 6 | 16% |
| Sports and Recreations | 6 | 16% |
| Arts and Humanities | 1 | 3% |
| Nursing and Health Professions | 1 | 3% |
| Other | 3 | 8% |
| Unknown | 11 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 October 2020.
All research outputs
#4,672,139
of 25,707,225 outputs
Outputs from Analytical & Bioanalytical Chemistry
#693
of 9,723 outputs
Outputs of similar age
#37,528
of 210,414 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#10
of 73 outputs
Altmetric has tracked 25,707,225 research outputs across all sources so far. Compared to these this one has done well and is in the 81st percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 9,723 research outputs from this source. They receive a mean Attention Score of 3.1. This one has done particularly well, scoring higher than 92% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 210,414 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 82% of its contemporaries.
We're also able to compare this research output to 73 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 86% of its contemporaries.