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Cytokines and microbicidal molecules regulated by IL-32 in THP-1-derived human macrophages infected with New World Leishmania species

Overview of attention for article published in PLoS Neglected Tropical Diseases, February 2017
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Title
Cytokines and microbicidal molecules regulated by IL-32 in THP-1-derived human macrophages infected with New World Leishmania species
Published in
PLoS Neglected Tropical Diseases, February 2017
DOI 10.1371/journal.pntd.0005413
Pubmed ID
Authors

Jéssica Cristina dos Santos, Bas Heinhuis, Rodrigo Saar Gomes, Michelle S. M. A. Damen, Fernando Real, Renato A. Mortara, Samuel T. Keating, Charles A. Dinarello, Leo A. B. Joosten, Fátima Ribeiro-Dias

Abstract

Interleukin-32 (IL-32) is expressed in lesions of patients with American Tegumentary Leishmaniasis (ATL), but its precise role in the disease remains unknown. In the present study, silencing and overexpression of IL-32 was performed in THP-1-derived macrophages infected with Leishmania (Viannia) braziliensis or L. (Leishmania) amazonensis to investigate the role of IL-32 in infection. We report that Leishmania species induces IL-32γ, and show that intracellular IL-32γ protein production is dependent on endogenous TNFα. Silencing or overexpression of IL-32 demonstrated that this cytokine is closely related to TNFα and IL-8. Remarkably, the infection index was augmented in the absence of IL-32 and decreased in cells overexpressing this cytokine. Mechanistically, these effects can be explained by nitric oxide cathelicidin and β-defensin 2 production regulated by IL-32. Thus, endogenous IL-32 is a crucial cytokine involved in the host defense against Leishmania parasites.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 75 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 75 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 12 16%
Student > Ph. D. Student 12 16%
Student > Bachelor 9 12%
Student > Doctoral Student 7 9%
Researcher 6 8%
Other 12 16%
Unknown 17 23%
Readers by discipline Count As %
Immunology and Microbiology 18 24%
Agricultural and Biological Sciences 11 15%
Biochemistry, Genetics and Molecular Biology 9 12%
Medicine and Dentistry 7 9%
Pharmacology, Toxicology and Pharmaceutical Science 5 7%
Other 6 8%
Unknown 19 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 February 2017.
All research outputs
#20,660,571
of 25,382,440 outputs
Outputs from PLoS Neglected Tropical Diseases
#8,185
of 9,377 outputs
Outputs of similar age
#252,427
of 325,414 outputs
Outputs of similar age from PLoS Neglected Tropical Diseases
#209
of 223 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
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We're also able to compare this research output to 223 others from the same source and published within six weeks on either side of this one. This one is in the 2nd percentile – i.e., 2% of its contemporaries scored the same or lower than it.