| Title |
Solid-state NMR sequential assignments of the amyloid core of full-length Sup35p
|
|---|---|
| Published in |
Biomolecular NMR Assignments, August 2013
|
| DOI | 10.1007/s12104-013-9515-1 |
| Pubmed ID | |
| Authors |
Anne K. Schütz, Birgit Habenstein, Nina Luckgei, Luc Bousset, Yannick Sourigues, Anders B. Nielsen, Ronald Melki, Anja Böckmann, Beat H. Meier |
| Abstract |
Sup35p is a yeast prion and is responsible for the [PSI (+)] trait in Saccharomyces cerevisiae. With 685 amino acids, full-length soluble and fibrillar Sup35p are challenging targets for structural biology as they cannot be investigated by X-ray crystallography or NMR in solution. We present solid-state NMR studies of fibrils formed by the full-length Sup35 protein. We detect an ordered and rigid core of the protein that gives rise to narrow and strong peaks, while large parts of the protein show either static disorder or dynamics on time scales which interfere with dipolar polarization transfer or shorten the coherence lifetime. Thus, only a small subset of resonances is observed in 3D spectra. Here we describe in detail the sequential assignments of the 22 residues for which resonances are observed in 3D spectra: their chemical shifts mostly corresponding to β-sheet secondary structure. We suspect that these residues form the amyloid core of the fibril. |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 28 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 25% |
| Researcher | 7 | 25% |
| Professor | 3 | 11% |
| Professor > Associate Professor | 3 | 11% |
| Student > Doctoral Student | 2 | 7% |
| Other | 1 | 4% |
| Unknown | 5 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 32% |
| Chemistry | 7 | 25% |
| Agricultural and Biological Sciences | 4 | 14% |
| Psychology | 1 | 4% |
| Unknown | 7 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 August 2013.
All research outputs
#20,198,525
of 22,716,996 outputs
Outputs from Biomolecular NMR Assignments
#180
of 276 outputs
Outputs of similar age
#172,196
of 196,389 outputs
Outputs of similar age from Biomolecular NMR Assignments
#2
of 3 outputs
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