| Title |
CRISPR/Cas9-mediated gene editing in human zygotes using Cas9 protein
|
|---|---|
| Published in |
Molecular Genetics and Genomics, March 2017
|
| DOI | 10.1007/s00438-017-1299-z |
| Pubmed ID | |
| Authors |
Lichun Tang, Yanting Zeng, Hongzi Du, Mengmeng Gong, Jin Peng, Buxi Zhang, Ming Lei, Fang Zhao, Weihua Wang, Xiaowei Li, Jianqiao Liu |
| Abstract |
Previous works using human tripronuclear zygotes suggested that the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system could be a tool in correcting disease-causing mutations. However, whether this system was applicable in normal human (dual pronuclear, 2PN) zygotes was unclear. Here we demonstrate that CRISPR/Cas9 is also effective as a gene-editing tool in human 2PN zygotes. By injection of Cas9 protein complexed with the appropriate sgRNAs and homology donors into one-cell human embryos, we demonstrated efficient homologous recombination-mediated correction of point mutations in HBB and G6PD. However, our results also reveal limitations of this correction procedure and highlight the need for further research. |
X Demographics
The data shown below were collected from the profiles of 105 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 14 | 13% |
| Japan | 10 | 10% |
| France | 6 | 6% |
| Canada | 5 | 5% |
| Saudi Arabia | 3 | 3% |
| United Kingdom | 3 | 3% |
| Germany | 2 | 2% |
| Mexico | 2 | 2% |
| Ireland | 1 | <1% |
| Other | 9 | 9% |
| Unknown | 50 | 48% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 72 | 69% |
| Scientists | 22 | 21% |
| Science communicators (journalists, bloggers, editors) | 8 | 8% |
| Practitioners (doctors, other healthcare professionals) | 3 | 3% |
Mendeley readers
The data shown below were compiled from readership statistics for 488 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Sweden | 1 | <1% |
| Finland | 1 | <1% |
| United Kingdom | 1 | <1% |
| Japan | 1 | <1% |
| United States | 1 | <1% |
| Unknown | 483 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 126 | 26% |
| Student > Master | 71 | 15% |
| Student > Ph. D. Student | 69 | 14% |
| Researcher | 48 | 10% |
| Other | 25 | 5% |
| Other | 55 | 11% |
| Unknown | 94 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 181 | 37% |
| Agricultural and Biological Sciences | 84 | 17% |
| Medicine and Dentistry | 27 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 14 | 3% |
| Chemistry | 13 | 3% |
| Other | 59 | 12% |
| Unknown | 110 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 366. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 December 2023.
All research outputs
#88,404
of 25,774,185 outputs
Outputs from Molecular Genetics and Genomics
#1
of 3,334 outputs
Outputs of similar age
#2,213
of 325,489 outputs
Outputs of similar age from Molecular Genetics and Genomics
#1
of 20 outputs
Altmetric has tracked 25,774,185 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,334 research outputs from this source. They receive a mean Attention Score of 3.9. This one has done particularly well, scoring higher than 99% of its peers.
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We're also able to compare this research output to 20 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.