Title |
Conditional targeted genome editing using somatically expressed TALENs in C. elegans
|
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Published in |
Nature Biotechnology, August 2013
|
DOI | 10.1038/nbt.2674 |
Pubmed ID | |
Authors |
Ze Cheng, Peishan Yi, Xiangming Wang, Yongping Chai, Guoxin Feng, Yihong Yang, Xing Liang, Zhiwen Zhu, Wei Li, Guangshuo Ou |
Abstract |
We have developed a method for the generation of conditional knockouts in Caenorhabditis elegans by expressing transcription activator-like effector nucleases (TALENs) in somatic cells. Using germline transformation with plasmids encoding TALENs under the control of an inducible or tissue-specific promoter, we observed effective gene modifications and resulting phenotypes in specific developmental stages and tissues. We further used this method to bypass the embryonic requirement of cor-1, which encodes the homolog of human severe combined immunodeficiency (SCID) protein coronin, and we determined its essential role in cell migration in larval Q-cell lineages. Our results show that TALENs expressed in the somatic cells of model organisms provide a versatile tool for functional genomics. |
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