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Mendeley readers
| Title |
Isoform- and receptor-specific channel property of canonical transient receptor potential (TRPC)1/4 channels
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|---|---|
| Published in |
Pflügers Archiv - European Journal of Physiology, August 2013
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| DOI | 10.1007/s00424-013-1332-y |
| Pubmed ID | |
| Authors |
Jinsung Kim, Misun Kwak, Jae-Pyo Jeon, Jongyun Myeong, Jinhong Wie, Chansik Hong, Sung-Young Kim, Ju-Hong Jeon, Hyun Jin Kim, Insuk So |
| Abstract |
Transient receptor potential canonical (TRPC) 1, the first mammalian homologue of Drosophila trp gene, is distributed widely in mammalian cells and is involved in many physiological functions. TRPC1 is reported to be functional following heteromeric formation with other TRPC channels such as TRPC4 or TRPC5. It is known that the composition of this widely distributed TRPC1 is far from simple; functionality of such channels has been highly controversial. Furthermore, TRPC1 gene is known to have two splicing variants; one encodes long (TRPC1α) and the other encodes short (TRPC1β) TRPC1 isoforms, respectively. In this study, we examined the functionality of TRPC1/4 channels using various activation systems. Gq/11-coupled receptor (e.g., M1 or M3 receptors) stimulation significantly increased TRPC1α/4 currents but induced mild activation of TRPC1β/4. In addition, when expressed with TRPC4, TRPC1α acted as a pore-constituting subunit and not a β ancillary subunit. Multimerized with TRPC4, TRPC1α also generated strong pore field strength. We also found that Gi/o-coupled receptor (e.g., M2 receptor) stimulation was insufficient to activate TRPC1α/4 and TRPC1β/4 channels but selectively activated TRPC4 homomeric channels. These findings demonstrate that TRPC1/4 channel shows dynamic gating property depending on TRPC1 isoform subtypes and receptor stimulation system. Therefore, careful discrimination of the specificity of TRPC1 isoforms and upstream activation system is important in thorough understanding of TRPC1 and TRPC1/4 channels. |
Mendeley readers
The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 3% |
| Unknown | 37 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 18% |
| Student > Ph. D. Student | 5 | 13% |
| Student > Master | 5 | 13% |
| Professor | 4 | 11% |
| Professor > Associate Professor | 4 | 11% |
| Other | 8 | 21% |
| Unknown | 5 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 34% |
| Biochemistry, Genetics and Molecular Biology | 8 | 21% |
| Neuroscience | 5 | 13% |
| Chemistry | 3 | 8% |
| Medicine and Dentistry | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 7 | 18% |