| Title |
Auxin synthesis gene tms1 driven by tuber-specific promoter alters hormonal status of transgenic potato plants and their responses to exogenous phytohormones
|
|---|---|
| Published in |
Plant Cell Reports, December 2016
|
| DOI | 10.1007/s00299-016-2091-y |
| Pubmed ID | |
| Authors |
Oksana O. Kolachevskaya, Lidiya I. Sergeeva, Kristyna Floková, Irina A. Getman, Sergey N. Lomin, Valeriya V. Alekseeva, Elena B. Rukavtsova, Yaroslav I. Buryanov, Georgy A. Romanov |
| Abstract |
Ectopic auxin overproduction in transgenic potato leads to enhanced productivity accompanied with concerted and occasional changes in hormonal status, and causing altered response of transformants to exogenous auxin or cytokinin. Previously, we generated potato transformants expressing Agrobacterium-derived auxin synthesis gene tms1 driven by tuber-specific patatin gene promoter (B33-promoter). Here, we studied the endogenous hormonal status and the response to exogenous phytohormones in tms1 transformants cultured in vitro. Adding indole-3-acetic acid (IAA) or kinetin to culture medium affected differently tuberization of tms1-transformed and control plants, depending also on sucrose content in the medium. Exogenous phytohormones ceased to stimulate the tuber initiation in transformants at high (5-8%) sucrose concentration, while in control plants the stimulation was observed in all experimental settings. Furthermore, exogenous auxin partly inhibited the tuber initiation, and exogenous cytokinin reduced the average tuber weight in most transformants at high sucrose content. The elevated auxin level in tubers of the transformants was accompanied with a decrease in content of cytokinin bases and their ribosides in tubers and most shoots. No concerted changes in contents of abscisic, jasmonic, salicylic acids and gibberellins in tubers were detected. The data on hormonal status indicated that the enhanced productivity of tms1 transformants was due to auxin and not mediated by other phytohormones. In addition, exogenous cytokinin was shown to upregulate the expression of genes encoding orthologs of auxin receptors. Overall, the results showed that tms1 expression and local increase in IAA level in transformants affect both the balance of endogenous cytokinins and the dynamics of tuberization in response to exogenous hormones (auxin, cytokinin), the latter reaction depending also on the carbohydrate supply. We introduce a basic model for the hormonal network controlling tuberization. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 25% |
| Mexico | 1 | 25% |
| Unknown | 2 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 3 | 75% |
| Members of the public | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 3% |
| India | 1 | 3% |
| Unknown | 32 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 26% |
| Researcher | 5 | 15% |
| Student > Postgraduate | 4 | 12% |
| Student > Master | 4 | 12% |
| Student > Doctoral Student | 2 | 6% |
| Other | 5 | 15% |
| Unknown | 5 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 17 | 50% |
| Biochemistry, Genetics and Molecular Biology | 8 | 24% |
| Energy | 1 | 3% |
| Unknown | 8 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 20. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 December 2020.
All research outputs
#1,563,566
of 22,958,253 outputs
Outputs from Plant Cell Reports
#35
of 2,192 outputs
Outputs of similar age
#34,663
of 421,291 outputs
Outputs of similar age from Plant Cell Reports
#1
of 36 outputs
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