Title |
Changes in the microbial community of an anammox consortium during adaptation to marine conditions revealed by 454 pyrosequencing
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Published in |
Applied Microbiology and Biotechnology, March 2017
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DOI | 10.1007/s00253-017-8160-5 |
Pubmed ID | |
Authors |
Blanca M. Gonzalez-Silva, Are J. Rønning, Ingrid K. Andreassen, Ingrid Bakke, Francisco J. Cervantes, Kjetill Østgaard, Olav Vadstein |
Abstract |
The anammox activity of a freshwater anammox consortium was strongly inhibited at low-salinity level. Stepwise adaptation from 0 to 3 g NaCl L(-1) took 153 days. Further adaptation to high-salinity concentration (from 3 to 30 g L(-1)) took only 40 days, and no inhibition was observed. A comprehensive insight into the salinity-induced successions of the total and the anammox communities was obtained by 454 pyrosequencing of 16S rRNA gene amplicons and statistical analysis. A major succession in the anammox community was observed at 3 g L(-1) where the dominating population shifted from Candidatus Brocadia fulgida to Ca. Kuenenia stuttgartiensis. The latter dominated at high salinity and seemed to be essential for the high (˃96%) ammonium and nitrite removal efficiencies achieved. SIMPER analysis indicated that these two dominating anammox species explained most to the differences in community structure among samples and helped in identifying other important members at different salinities. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Netherlands | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
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Unknown | 78 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 13 | 17% |
Student > Master | 10 | 13% |
Student > Bachelor | 8 | 10% |
Student > Doctoral Student | 7 | 9% |
Student > Ph. D. Student | 7 | 9% |
Other | 8 | 10% |
Unknown | 25 | 32% |
Readers by discipline | Count | As % |
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Engineering | 12 | 15% |
Biochemistry, Genetics and Molecular Biology | 5 | 6% |
Agricultural and Biological Sciences | 4 | 5% |
Chemical Engineering | 2 | 3% |
Other | 5 | 6% |
Unknown | 29 | 37% |