| Title |
Generation of stable nulliplex autopolyploid lines of Arabidopsis thaliana using CRISPR/Cas9 genome editing
|
|---|---|
| Published in |
Plant Cell Reports, March 2017
|
| DOI | 10.1007/s00299-017-2125-0 |
| Pubmed ID | |
| Authors |
Peter Ryder, Marcus McHale, Antoine Fort, Charles Spillane |
| Abstract |
RNA-guided endonuclease-mediated targeted mutagenesis using the clustered regularly interspersed short palindromic repeats (CRISPR)/Cas9 system has been successful at targeting specific loci for modification in plants. While polyploidy is an evolutionary mechanism enabling plant adaptation, the analysis of gene function in polyploid plants has been limited due to challenges associated with generating polyploid knockout mutants for all gene copies in polyploid plant lines. This study investigated whether CRISPR/Cas9 mediated targeted mutagenesis can generate nulliplex tetraploid mutant lines in Arabidopsis thaliana, while also comparing the relative efficiency of targeted mutagenesis in tetraploid (4x) versus diploid (2x) backgrounds. Using CRISPR/Cas9 genome editing to generate knockout alleles of the TTG1 gene, we demonstrate that homozygous nulliplex mutants can be directly generated in tetraploid Arabidopsis thaliana plants. CRISPR/Cas9 genome editing now provides a route to more efficient generation of polyploid mutants for improving understanding of genome dosage effects in plants. |
X Demographics
The data shown below were collected from the profiles of 19 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 2 | 11% |
| Italy | 2 | 11% |
| Brazil | 1 | 5% |
| Peru | 1 | 5% |
| Belgium | 1 | 5% |
| Mexico | 1 | 5% |
| Canada | 1 | 5% |
| Germany | 1 | 5% |
| United Kingdom | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 7 | 37% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 13 | 68% |
| Scientists | 6 | 32% |
Mendeley readers
The data shown below were compiled from readership statistics for 53 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Chile | 1 | 2% |
| Netherlands | 1 | 2% |
| Italy | 1 | 2% |
| Argentina | 1 | 2% |
| Unknown | 49 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 16 | 30% |
| Researcher | 14 | 26% |
| Other | 4 | 8% |
| Student > Bachelor | 3 | 6% |
| Professor | 3 | 6% |
| Other | 6 | 11% |
| Unknown | 7 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 29 | 55% |
| Biochemistry, Genetics and Molecular Biology | 11 | 21% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
| Computer Science | 1 | 2% |
| Sports and Recreations | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 9 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 August 2020.
All research outputs
#3,254,345
of 23,592,647 outputs
Outputs from Plant Cell Reports
#138
of 2,234 outputs
Outputs of similar age
#60,791
of 309,554 outputs
Outputs of similar age from Plant Cell Reports
#3
of 48 outputs
Altmetric has tracked 23,592,647 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,234 research outputs from this source. They receive a mean Attention Score of 4.1. This one has done particularly well, scoring higher than 93% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 309,554 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 48 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.