| Title |
Advances in glycosaminoglycanomics by 15N-NMR spectroscopy
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, February 2013
|
| DOI | 10.1007/s00216-013-6803-7 |
| Pubmed ID | |
| Authors |
Vitor H. Pomin |
| Abstract |
Recent developments to enhance sensitivity in solution NMR spectroscopy such as the advent and spread of the use of high magnetic fields, cryoprobe technology, isotopic labeling techniques, and new combinations of 2D experiments have pushed the limits in structural NMR analysis of glycosaminoglycans (GAGs). This review is dedicated to the less sensitive (15)N isotope of hexosamines rather than the commonly used anomeric and ring (1)H and (13)C resonances of uronic acids and hexosamines. Given that GAG types are basically classified on the basis of their composing hexosamine types together with variations of their sulfation patterns, and epimerized forms of the adjacent uronic acids, (15)N-related NMR studies on native GAGs, oligosaccharides, or the various composing amino sugars have proved to be quite useful in the retrieval of both structural and dynamic information, despite the low number of resultant peaks. This in turn reduces significantly chemical shift degeneracy and at the same time facilitates spin and structural assignments. This review covers the principal contributions made so far by solution (15)N-NMR spectroscopy to progress in the structural biology of GAGs in the current glycomics age. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 19 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 37% |
| Student > Doctoral Student | 1 | 5% |
| Professor | 1 | 5% |
| Other | 1 | 5% |
| Student > Master | 1 | 5% |
| Other | 3 | 16% |
| Unknown | 5 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 7 | 37% |
| Biochemistry, Genetics and Molecular Biology | 2 | 11% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 11% |
| Agricultural and Biological Sciences | 2 | 11% |
| Medicine and Dentistry | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 5 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 September 2013.
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#17,286,379
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Outputs from Analytical & Bioanalytical Chemistry
#5,669
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#131,599
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Outputs of similar age from Analytical & Bioanalytical Chemistry
#52
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