Title |
Zscan4 is expressed specifically during late meiotic prophase in both spermatogenesis and oogenesis
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Published in |
In Vitro Cellular & Developmental Biology - Animal, October 2016
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DOI | 10.1007/s11626-016-0096-z |
Pubmed ID | |
Authors |
Kei-ichiro Ishiguro, Manuela Monti, Tomohiko Akiyama, Hiromi Kimura, Nana Chikazawa-Nohtomi, Miki Sakota, Saeko Sato, Carlo Alberto Redi, Shigeru B. H. Ko, Minoru S. H. Ko |
Abstract |
Mouse zinc finger and SCAN domain containing 4 (Zscan4) proteins, which are encoded by multiple copies of Zscan4 genes, are expressed specifically in preimplantation embryos in vivo and embryonic stem (ES) cells in vitro. However, the expression patterns of mouse Zscan4 in vivo have been largely elusive. Here, we show that Zscan4 proteins are expressed in adult ovaries and testes. In ovaries, Zscan4 proteins were detected in germinal vesicle (GV) stage oocytes in antral follicles, indicating that Zscan4 genes are activated during the diplotene/dictyate stage in meiotic prophase I. Remarkably, Zscan4 showed different spatial localization patterns between two distinct GV oocytes, which can be distinguished by global chromatin organization-surrounded nucleolus (SN) and non-surrounded nucleolus (NSN). These spatiotemporal differences in Zscan4 localizations correlated with the transition of RNA polymerase II-mediated transcriptional status during GV oocyte maturation. In testes, Zscan4 proteins were detected in spermatocytes at late pachytene/diplotene stages and in Sertoli cells. These results suggest that Zscan4 may play critical roles during late meiotic prophase in both males and females. |
X Demographics
Geographical breakdown
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United States | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 33 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 5 | 15% |
Researcher | 5 | 15% |
Student > Doctoral Student | 4 | 12% |
Student > Master | 4 | 12% |
Professor | 2 | 6% |
Other | 3 | 9% |
Unknown | 10 | 30% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 6 | 18% |
Unspecified | 1 | 3% |
Veterinary Science and Veterinary Medicine | 1 | 3% |
Medicine and Dentistry | 1 | 3% |
Other | 0 | 0% |
Unknown | 11 | 33% |