| Chapter title |
DNA-Directed Antibody Immobilization for Robust Protein Microarrays: Application to Single Particle Detection ‘DNA-Directed Antibody Immobilization
|
|---|---|
| Chapter number | 12 |
| Book title |
Biosensors and Biodetection
|
| Published in |
Methods in molecular biology, March 2017
|
| DOI | 10.1007/978-1-4939-6848-0_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6846-6, 978-1-4939-6848-0
|
| Authors |
Nese Lortlar Ünlü, Fulya Ekiz Kanik, Elif Seymour, John H. Connor, M. Selim Ünlü |
| Editors |
Avraham Rasooly, Ben Prickril |
| Abstract |
Protein microarrays are emerging tools which have become very powerful in multiplexed detection technologies. A variety of proteins can be immobilized on a sensor chip allowing for multiplexed diagnostics. Therefore, various types of analyte in a small volume of sample can be detected simultaneously. Protein immobilization is a crucial step for creating a robust and sensitive protein microarray-based detection system. In order to achieve a successful protein immobilization and preserve the activity of the proteins after immobilization, DNA-directed immobilization is a promising technique. Here, we present the design and the use of DNA-directed immobilized (DDI) antibodies in fabrication of robust protein microarrays. We focus on application of protein microarrays for capturing and detecting nanoparticles such as intact viruses. Experimental results on Single-particle interferometric reflectance imaging sensor (SP-IRIS) are used to validate the advantages of the DDI method. |
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