| Title |
Determination of true ratios of different N-glycan structures in electrospray ionization mass spectrometry
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, March 2017
|
| DOI | 10.1007/s00216-017-0235-8 |
| Pubmed ID | |
| Authors |
Clemens Grünwald-Gruber, Andreas Thader, Daniel Maresch, Thomas Dalik, Friedrich Altmann |
| Abstract |
An ideal method for the analysis of N-glycans would both identify the isomeric structure and deliver a true picture of the relative, if not absolute, amounts of the various structures in one sample. Porous graphitic carbon chromatography coupled with electrospray ionization mass spectrometry (ESI-MS) detection has emerged as a method with a particularly high potential of resolving isomeric oligosaccharides, but little attention has so far been paid to quantitation of the results obtained. In this work, we isolated a range of structures from Man5 to complex type N-glycans with zero to four sialic acids and blended them into an equimolar "glyco tune mix". When subjected to liquid chromatography-ESI-MS in positive and negative modes, the glyco tune mix clearly demonstrated the futility of quantitation of N-glycans of different overall composition, different number of sialic acids, and strongly differing size without compensation for their very different molar responses. Relative quantitation of human plasma N-glycans was performed with correction factors deduced from this external glyco tune mix. Addition of just one isotope-coded internal standard with enzymatically added (13)C-galactose led to absolute quantification in the same experiment. Graphical Abstract Discrepancy between desirable (grey bars) and real (green bars) relative ion abundance of equimolar amounts of glycans in positive mode ESI-MS. |
X Demographics
The data shown below were collected from the profiles of 10 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 3 | 30% |
| United States | 1 | 10% |
| United Kingdom | 1 | 10% |
| Unknown | 5 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 6 | 60% |
| Members of the public | 3 | 30% |
| Science communicators (journalists, bloggers, editors) | 1 | 10% |
Mendeley readers
The data shown below were compiled from readership statistics for 49 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 49 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 13 | 27% |
| Student > Bachelor | 8 | 16% |
| Researcher | 7 | 14% |
| Student > Doctoral Student | 4 | 8% |
| Student > Postgraduate | 3 | 6% |
| Other | 5 | 10% |
| Unknown | 9 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 13 | 27% |
| Chemistry | 10 | 20% |
| Agricultural and Biological Sciences | 7 | 14% |
| Medicine and Dentistry | 3 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 4% |
| Other | 3 | 6% |
| Unknown | 11 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 March 2017.
All research outputs
#6,226,449
of 25,394,764 outputs
Outputs from Analytical & Bioanalytical Chemistry
#1,423
of 9,624 outputs
Outputs of similar age
#92,709
of 321,188 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#13
of 165 outputs
Altmetric has tracked 25,394,764 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 9,624 research outputs from this source. They receive a mean Attention Score of 3.1. This one has done well, scoring higher than 85% of its peers.
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We're also able to compare this research output to 165 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.