| Title |
In vitro comparative assessment of different viability assays in Acanthamoeba castellanii and Acanthamoeba polyphaga trophozoites
|
|---|---|
| Published in |
Parasitology Research, September 2013
|
| DOI | 10.1007/s00436-013-3599-5 |
| Pubmed ID | |
| Authors |
I. Heredero-Bermejo, J. L. Copa-Patiño, J. Soliveri, R. Gómez, F. J. de la Mata, J. Pérez-Serrano |
| Abstract |
The species of the genus Acanthamoeba are opportunistic protozoan parasites that cause different diseases in humans, such as amoebic keratitis and granulomatous encephalitis. The rise in the rate of Acanthamoeba keratitis, mainly due to the increase in contact lens wearers, turns the development of viability assays using a multi-well plate reader as a tool for screening new antiamoebic agents in vitro into an important goal. In our study, the viability assays PrestoBlue®, resazurin sodium salt, 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) and CellTiter96® were tested for their suitability as time-saving alternatives to the classical manual or direct-counting method, assessing the effect of the antiamoebic agent chlorhexidine digluconate and temperature on Acanthamoeba castellanii (ATCC® 30234™) and Acanthamoeba polyphaga 2961. Although resazurin and MTT have already been previously used in amoeba viability assays to test the activities of antiamoebic agents in vitro, it is the first time that PrestoBlue® and CellTiter96® are used for this purpose. Results indicated that the viability assays were strain-dependent leading in some cases to an overestimation of the real situation of viable cells. This implies that each viability assay ought to be set up for each amoeba strain studied. |
Mendeley readers
The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Portugal | 1 | 2% |
| Unknown | 59 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 13 | 22% |
| Student > Bachelor | 8 | 13% |
| Researcher | 7 | 12% |
| Student > Ph. D. Student | 6 | 10% |
| Student > Postgraduate | 3 | 5% |
| Other | 9 | 15% |
| Unknown | 14 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 22% |
| Biochemistry, Genetics and Molecular Biology | 7 | 12% |
| Medicine and Dentistry | 5 | 8% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 7% |
| Immunology and Microbiology | 4 | 7% |
| Other | 7 | 12% |
| Unknown | 20 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 September 2013.
All research outputs
#5,850,196
of 22,721,584 outputs
Outputs from Parasitology Research
#393
of 3,775 outputs
Outputs of similar age
#49,652
of 198,485 outputs
Outputs of similar age from Parasitology Research
#5
of 38 outputs
Altmetric has tracked 22,721,584 research outputs across all sources so far. This one has received more attention than most of these and is in the 74th percentile.
So far Altmetric has tracked 3,775 research outputs from this source. They receive a mean Attention Score of 2.7. This one has done well, scoring higher than 89% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 198,485 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.
We're also able to compare this research output to 38 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 81% of its contemporaries.