| Title |
Loss of SS18-SSX1 Inhibits Viability and Induces Apoptosis in Synovial Sarcoma
|
|---|---|
| Published in |
Clinical Orthopaedics & Related Research, May 2013
|
| DOI | 10.1007/s11999-013-3065-9 |
| Pubmed ID | |
| Authors |
Emily E. Carmody Soni, Silke Schlottman, Hayriye V. Erkizan, Aykut Uren, Jeffrey A. Toretsky |
| Abstract |
Most synovial sarcomas contain a chromosomal translocation t(X;18), which results in the formation of an oncoprotein SS18-SSX critical to the viability of synovial sarcoma. We (1) established and characterized three novel synovial sarcoma cell lines and asked (2) whether inhibition of SS18-SSX1 decreases cell viability in these cell lines; and (3) whether reduction in viability after SS18-SSX1 knockdown is caused by apoptosis. After identifying a specific posttranscriptional splice variant in our cell lines, we asked (4) whether this provides a survival benefit in synovial sarcoma. Cells lines were characterized. SS18-SSX1 knockdown was achieved using a shRNA system. Cell viability was assessed by WST-1 analysis and apoptosis examined by caspase-3 activity. We confirmed the SS18-SSX1 translocation in all cell lines and identified a consistent splicing variant. We achieved successful knockdown of SS18-SSX1 and with this saw a significant reduction in cell viability. Decreased viability was a result of increased apoptosis. Reintroduction of the exon 8 sequence into cells reduced cell viability in all cell lines. We confirmed the presence of the SS18-SSX1 translocation in our cell lines and its importance in the survival of synovial sarcoma. We have also demonstrated that reduction in cell viability is related to an increase in apoptosis. In addition, we have identified a potential mediator of SS18-SSX function in exon 8. SS18-SSX represents a tumor-specific target in synovial sarcoma. Exploitation of SS18-SSX and its protein partners will allow us to develop potent tumor-specific therapeutic agents. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 5% |
| Unknown | 19 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 30% |
| Student > Bachelor | 3 | 15% |
| Student > Master | 3 | 15% |
| Student > Ph. D. Student | 2 | 10% |
| Student > Doctoral Student | 2 | 10% |
| Other | 2 | 10% |
| Unknown | 2 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 30% |
| Agricultural and Biological Sciences | 4 | 20% |
| Medicine and Dentistry | 4 | 20% |
| Psychology | 1 | 5% |
| Arts and Humanities | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 4 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 July 2013.
All research outputs
#22,758,309
of 25,371,288 outputs
Outputs from Clinical Orthopaedics & Related Research
#6,736
of 7,298 outputs
Outputs of similar age
#182,594
of 207,671 outputs
Outputs of similar age from Clinical Orthopaedics & Related Research
#110
of 129 outputs
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