| Title |
Improvement of vitamin E quality and quantity in tobacco and lettuce by chloroplast genetic engineering
|
|---|---|
| Published in |
Transgenic Research, September 2012
|
| DOI | 10.1007/s11248-012-9656-5 |
| Pubmed ID | |
| Authors |
Yukinori Yabuta, Hiroyuki Tanaka, Sahoko Yoshimura, Akiko Suzuki, Masahiro Tamoi, Takanori Maruta, Shigeru Shigeoka |
| Abstract |
Vitamin E (tocopherol: Toc) is an important lipid-soluble antioxidant synthesized in chloroplasts. Among the 8 isoforms of vitamin E, α-Toc has the highest activity in humans. To generate transgenic plants with enhanced vitamin E activity, we applied a chloroplast transformation technique. Three types of the transplastomic tobacco plants (pTTC, pTTMT and pTTC-TMT) carrying the Toc cyclase (TC) or γ-Toc methyltransferase (γ-TMT) gene and the TC plus γ-TMT genes as an operon in the plastid genome, respectively, were generated. There was a significant increase in total levels of Toc due to an increase in γ-Toc in the pTTC plants. Compared to the wild-type plants, Toc composition was altered in the pTTMT plants. In the pTTC-TMT plants, total Toc levels increased and α-Toc was a major Toc isoform. Furthermore, to use chloroplast transformation to produce α-Toc-rich vegetable, TC-overexpressing transplastomic lettuce plants (pLTC) were generated. Total Toc levels and vitamin E activity increased in the pLTC plants compared with the wild-type lettuce plants. These findings indicated that chloroplast genetic engineering is useful to improve vitamin E quality and quantity in plants. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 64 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 2 | 3% |
| France | 1 | 2% |
| Greece | 1 | 2% |
| Unknown | 60 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 17 | 27% |
| Student > Master | 7 | 11% |
| Student > Doctoral Student | 6 | 9% |
| Researcher | 5 | 8% |
| Student > Bachelor | 4 | 6% |
| Other | 11 | 17% |
| Unknown | 14 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 35 | 55% |
| Biochemistry, Genetics and Molecular Biology | 5 | 8% |
| Earth and Planetary Sciences | 2 | 3% |
| Computer Science | 1 | 2% |
| Economics, Econometrics and Finance | 1 | 2% |
| Other | 3 | 5% |
| Unknown | 17 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 June 2015.
All research outputs
#3,720,274
of 22,721,584 outputs
Outputs from Transgenic Research
#155
of 890 outputs
Outputs of similar age
#26,752
of 170,624 outputs
Outputs of similar age from Transgenic Research
#2
of 10 outputs
Altmetric has tracked 22,721,584 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 890 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one has done well, scoring higher than 82% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 170,624 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 84% of its contemporaries.
We're also able to compare this research output to 10 others from the same source and published within six weeks on either side of this one. This one has scored higher than 8 of them.