| Title |
Elevated blood Hsp60, its structural similarities and cross-reactivity with thyroid molecules, and its presence on the plasma membrane of oncocytes point to the chaperonin as an immunopathogenic factor in Hashimoto's thyroiditis
|
|---|---|
| Published in |
Cell Stress and Chaperones, September 2013
|
| DOI | 10.1007/s12192-013-0460-9 |
| Pubmed ID | |
| Authors |
Antonella Marino Gammazza, Manfredi Rizzo, Roberto Citarrella, Francesca Rappa, Claudia Campanella, Fabio Bucchieri, Angelo Patti, Dragana Nikolic, Daniela Cabibi, Giandomenico Amico, Pier Giulio Conaldi, Pier Luigi San Biagio, Giuseppe Montalto, Felicia Farina, Giovanni Zummo, Everly Conway de Macario, Alberto J.L. Macario, Francesco Cappello |
| Abstract |
The role Hsp60 might play in various inflammatory and autoimmune diseases is under investigation, but little information exists pertaining to Hashimoto's thyroiditis (HT). With the aim to fill this gap, in the present work, we directed our attention to Hsp60 participation in HT pathogenesis. We found Hsp60 levels increased in the blood of HT patients compared to controls. The chaperonin was immunolocalized in thyroid tissue specimens from patients with HT, both in thyrocytes and oncocytes (Hurthle cells) with higher levels compared to controls (goiter). In oncocytes, we found Hsp60 not only in the cytoplasm but also on the plasma membrane, as shown by double immunofluorescence performed on fine needle aspiration cytology. By bioinformatics, we found regions in the Hsp60 molecule with remarkable structural similarity with the thyroglobulin (TG) and thyroid peroxidase (TPO) molecules, which supports the notion that autoantibodies against TG and TPO are likely to recognize Hsp60 on the plasma membrane of oncocytes. This was also supported by data obtained by ELISA, showing that anti-TG and anti-TPO antibodies cross-react with human recombinant Hsp60. Antibody-antigen (Hsp60) reaction on the cell surface could very well mediate thyroid cell damage and destruction, perpetuating inflammation. Experiments with recombinant Hsp60 did not show stimulation of cytokine production by peripheral blood mononuclear cells from HT patients. All together, these results led us to hypothesize that Hsp60 may be an active player in HT pathogenesis via an antibody-mediated immune mechanism. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Italy | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 3% |
| Unknown | 32 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Postgraduate | 5 | 15% |
| Student > Master | 4 | 12% |
| Other | 3 | 9% |
| Researcher | 3 | 9% |
| Student > Ph. D. Student | 2 | 6% |
| Other | 5 | 15% |
| Unknown | 11 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 10 | 30% |
| Agricultural and Biological Sciences | 5 | 15% |
| Psychology | 2 | 6% |
| Physics and Astronomy | 1 | 3% |
| Engineering | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 13 | 39% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 September 2013.
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#20,653,708
of 25,371,288 outputs
Outputs from Cell Stress and Chaperones
#494
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#161,393
of 214,033 outputs
Outputs of similar age from Cell Stress and Chaperones
#7
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