Title |
Solution NMR structure of CD1104B from pathogenic Clostridium difficile reveals a distinct α-helical architecture and provides first structural representative of protein domain family PF14203
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Published in |
Journal of Structural and Functional Genomics, September 2013
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DOI | 10.1007/s10969-013-9164-8 |
Pubmed ID | |
Authors |
Surya V. S. R. K. Pulavarti, Alexander Eletsky, Hsiau-Wei Lee, Thomas B. Acton, Rong Xiao, John K. Everett, James H. Prestegard, Gaetano T. Montelione, Thomas Szyperski |
Abstract |
A high-quality structure of the 68-residue protein CD1104B from Clostridium difficile strain 630 exhibits a distinct all α-helical fold. The structure presented here is the first representative of bacterial protein domain family PF14203 (currently 180 members) of unknown function (DUF4319) and reveals that the side-chains of the only two strictly conserved residues (Glu 8 and Lys 48) form a salt bridge. Moreover, these two residues are located in the vicinity of the largest surface cleft which is predicted to contribute to a surface area involved in protein-protein interactions. This, along with its coding in transposon CTn4, suggests that CD1104B (and very likely all members of Pfam 14203) functions by interacting with other proteins required for the transfer of transposons between different bacterial species. |
X Demographics
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Unknown | 1 | 100% |
Demographic breakdown
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Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 2 | 20% |
Professor > Associate Professor | 2 | 20% |
Professor | 1 | 10% |
Researcher | 1 | 10% |
Student > Bachelor | 1 | 10% |
Other | 0 | 0% |
Unknown | 3 | 30% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 1 | 10% |
Agricultural and Biological Sciences | 1 | 10% |
Immunology and Microbiology | 1 | 10% |
Physics and Astronomy | 1 | 10% |
Earth and Planetary Sciences | 1 | 10% |
Other | 2 | 20% |
Unknown | 3 | 30% |